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Bactericidal composition containing validamycin and application thereof

A technology of Jinggangmycin and composition, which is applied in the field of bactericidal composition containing Jinggangmycin, can solve the problems of high toxicity of chemical pesticides, impact on food safety, and short duration of effect, and achieve obvious synergistic effect and improve drug efficacy , Improve the effect of duration

Inactive Publication Date: 2019-11-05
ZHEJIANG TONGLU HUIFENG BIOSCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] As biopesticides, Jinggangmycin and Bacillus tequila have the advantage of low toxicity, but their disadvantages are also obvious. Jinggangmycin has a short duration of efficacy in preventing and controlling plant diseases, and Bacillus tequila has poor efficacy in preventing and controlling plant diseases. , the share of chemical pesticides in the agents for preventing and treating plant diseases is increasing, but the toxicity of chemical pesticides is higher than that of biological pesticides, which affects food safety. Therefore, it is urgent to develop a biological fungicide with good effect, low toxicity and long duration

Method used

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  • Bactericidal composition containing validamycin and application thereof
  • Bactericidal composition containing validamycin and application thereof
  • Bactericidal composition containing validamycin and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Indoor Toxicity Test of Jinggangmycin Complex against Cucumber Powdery Mildew

[0031] Among them, the cucumber powdery mildew pathogen was collected from the field and purified and cultivated indoors.

[0032] Test method: After pre-testing to determine the effective inhibitory concentration range of each agent, Bacillus tequila (the effective number of viable bacteria of Bacillus tequila is above 100 billion cfu / g), Jinggangmycin and their mixed preparations The active ingredient content was serially diluted to 5 concentrations, and sterile water was used as a control, and the method of mycelium growth rate was used to measure. Add different concentrations of agents into sterilized petri dishes, 1 mL per dish, and then add 9 mL of sterilized and melted PDA medium , fully mixed to make a plate, and then the expanded colony reached a 0.5cm bacterial cake, one plate per plate, repeated 3 times per treatment, placed in a 28°C incubator for cultivation, and cultivated for ...

Embodiment 2

[0039] Combine Jinggangmycin and Bacillus tequila (the effective number of viable bacteria of Bacillus tequila is 100 billion cfu / g) in a weight ratio of 15:1, and carry out the field test of cucumber powdery mildew control, as shown in the following table Requirements, make different medicaments into corresponding concentrations, apply the medicine for the first time during the fruit-setting period of cucumbers, and apply the medicine for the second time 7 days later. efficacy.

[0040] Table 2 Comparison of the effects of pesticides for the control of cucumber powdery mildew

[0041]

[0042] (The data comes from the field efficacy test in 2018)

[0043] As can be seen from Table 2, the results of the field drug efficacy test show that under the test conditions of Example 2, the composition of Jinggangmycin and Bacillus tequila has excellent control effects on cucumber powdery mildew, and the control effect is comparable to that of a single agent. The ratio has a synerg...

Embodiment 3

[0045] Combine Jinggangmycin and Bacillus tequila (the effective number of viable bacteria of Bacillus tequila is 100 billion cfu / g) in a weight ratio of 5:1, and carry out a field test of cucumber powdery mildew control, as shown in the following table Requirements, make different medicaments into corresponding concentrations, apply the medicine for the first time during the fruit-setting period of cucumbers, and apply the medicine for the second time 7 days later. efficacy.

[0046] Table 3 Comparison of the effects of pesticides for the control of cucumber powdery mildew

[0047]

[0048] (The data comes from the field efficacy test in 2018)

[0049] As can be seen from Table 3, the results of the field efficacy test show that under the test conditions of Example 3, the composition of Jinggangmycin and Bacillus tequila has excellent control effects on cucumber powdery mildew, and the control effect is comparable to that of a single agent. The ratio has a synergistic effe...

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Abstract

The invention provides a bactericidal composition containing validamycin and belongs to the technical field of agricultural prevention and control. The compound includes validamycin and bacillus tequila, wherein the mass ratio of the validamycin to the bacillus tequila is (1-15):(1-15), and the number of effective viable bacteria of the bacillus tequila is 10 million cfu / g or above. The composition has an obvious synergistic effect after the validamycin and the bacillus tequila are mixed, the pesticide effect can be substantially improved, and the pesticide usage and the pesticide applicationcost are reduced. Meanwhile, the provided bactericidal composition can prolong the lasting period of the validamycin.

Description

technical field [0001] The invention belongs to the technical field of agricultural control, and in particular relates to a bactericidal composition containing Jinggangmycin and its application. Background technique [0002] Jinggangmycin, also known as effective mycin, is an antibiotic produced by actinomycetes. It has strong systemic properties, is easily absorbed by bacterial cells and conducts rapidly within it, interfering with and inhibiting the growth and development of bacterial cells. It is a low toxicity fungicide. Acute oral LD ​​in rats and mice 50 All greater than 2000 mg / kg, subcutaneous injection of LD 50 All greater than 1500 mg / kg. 5000 mg / kg applied to the skin of rats has no toxic reaction. Low toxicity to fish, carp TLM(96h)LD 50 >40 mg / L. [0003] Bacillus Tequila, a biocontrol bacterium obtained from the ocean. Bacillus Tequila can not only effectively inhibit the growth of pathogenic bacteria, but also produce compounds that stimulate plant g...

Claims

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Application Information

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IPC IPC(8): A01N43/16A01N63/00A01P3/00
CPCA01N43/16A01N63/00
Inventor 殷红福李忠王佳倪烈
Owner ZHEJIANG TONGLU HUIFENG BIOSCI
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