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Hg2+ detection method based on molecular beacon aptamer

A technology of molecular beacons and detection methods, applied in the direction of measuring devices, analytical materials, fluorescence/phosphorescence, etc., can solve the problems affecting the timeliness of Hg2+, cumbersome detection steps, etc. Effect

Inactive Publication Date: 2019-10-22
GUANGDONG UNIV OF PETROCHEMICAL TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Current Hg 2+ There are many kinds of detection methods, mainly including atomic absorption spectroscopy, atomic emission spectroscopy, inductively coupled plasma mass spectrometry and electrochemical methods, but these methods require the operation of professionals and large detection machines, and there are relatively cumbersome The detection step greatly affects the Hg 2+ Timeliness of detection

Method used

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  • Hg2+ detection method based on molecular beacon aptamer
  • Hg2+ detection method based on molecular beacon aptamer
  • Hg2+ detection method based on molecular beacon aptamer

Examples

Experimental program
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Embodiment 1

[0027] A Molecular Beacon Aptamer Based Hg 2+ detection method, the Hg 2+ The detection method comprises the following steps:

[0028] (1) Design of molecular beacon aptamer: design a single-stranded DNA probe composed of 29 bases, wherein the DNA probe contains a stem-loop hairpin structure close to the 5' end and the 3' end, and the 5' The end is marked with FAM, and the 3' end is marked with DABCYL;

[0029] (2) Preparation of buffer system: the preparation pH is 8.1, Na + The concentration is 40mmol / L, Mg 2+ The buffer system with a concentration of 10mmol / L is for use;

[0030] (3) The establishment of the control group and the experimental group: add a certain concentration of the above-mentioned molecular beacon aptamer to the buffer system, and then add the Hg-containing aptamer to be detected 2+ Solution, mixed to form an experimental group for later use, adding molecular beacon aptamers with the same concentration and volume as the experimental group to the buff...

Embodiment 2

[0037] Measure the inventive method Hg 2+ recovery rate:

[0038] (1) Select Hg 2+ The water sample with a content of 0.4 μmol / L is the test water sample. The test water sample is divided into three groups, each group is 1 L, and 8 μmol, 4 μmol and 0.8 μmol of Hg are added respectively. 2+ , mix well and set aside;

[0039] (2) adopt the detection method of embodiment 1 to detect its Hg respectively by above-mentioned each group of water sample 2+ The measured value, calculated the recovery, the results are shown in the following table:

[0040]

Embodiment 3

[0042] Using the detection method described in Example 1, replace the Hg containing Hg to be detected respectively. 2+ The solution contains Zn 2+ solution, containing Al 3+ solution, containing Mn 2+ solution, containing Sn 2+ solution, containing Co 2+ solution, containing K + solution, containing Cu 2+ solution, containing Ba 2+ solution, containing Ca 2+ solution and containing Cr 2+ Solution, to measure the influence of different metal ions on the fluorescence intensity of molecular beacons, the results are as follows Figure 4 shown.

[0043] It can be seen from the foregoing examples that the detection method of the present invention can effectively detect Hg in the solution. 2+ content, and the impact of other metal ions on the detection is low, the detection accuracy is high, and it is suitable for popularization and use.

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Abstract

The invention provides an Hg2+ detection method based on a molecular beacon aptamer. The Hg2+ detection method relates to the technical field of Hg2+ detection, and mainly comprises the steps of designing the molecular beacon aptamer, preparing a buffer system, setting up a control group and an experimental group, carrying out detection, establishing a standard curve, performing quantitative analysis and the like. The invention overcomes the deficiencies of the prior art, adopts the principle that a T-Hg2+-T structure is formed by a T base in the molecular beacon aptamer and Hg2+, the conformation change of the aptamer is triggered by means of the specific binding of the aptamer and Hg2+, thus a distance between fluorophore and a quenching group is widened and the fluorescence intensity isenhanced, enhances the sensitivity of detection, facilitates the detection personnel to observe and detect, is simple in preparation of solutions during the detection process, is convenient to use, reduces manpower and material resources, and is suitable for popularization and application.

Description

technical field [0001] The present invention relates to Hg 2+ Detection technology field, specifically related to a kind of Hg based on molecular beacon aptamer 2+ Detection method. Background technique [0002] Hg 2+ It is the ionic form of mercury, and mercury compounds are indispensable in many ways in modern industry. In recent years, due to the rapid development of people in industry, there are countless incidents of mercury poisoning. Hg 2+ Due to strong toxicity and great damage to the body, it develops fast and convenient Hg 2+ The detection method is particularly urgent. Current Hg 2+ There are many kinds of detection methods, mainly including atomic absorption spectroscopy, atomic emission spectroscopy, inductively coupled plasma mass spectrometry and electrochemical methods, but these methods require the operation of professionals and large detection machines, and there are relatively cumbersome The detection step greatly affects the Hg 2+ Timeliness of d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 张强王丽迟恩忠
Owner GUANGDONG UNIV OF PETROCHEMICAL TECH
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