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Analytical method of CREB in Ang-2 regulating VEGFR2 expression

An analysis method, ang-2 technology, applied in the biological field, can solve the problems of little analysis, lack of theoretical and clinical research, etc., to achieve the effect of accurate acquisition and improve the accuracy of image processing

Inactive Publication Date: 2019-10-15
THE FIRST AFFILIATED HOSPITAL OF ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] To sum up, the problems existing in the prior art are: domestic and foreign analysis on CREB in Ang-2 regulation of VEGFR2 expression is very little, lack of theoretical and clinical research

Method used

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  • Analytical method of CREB in Ang-2 regulating VEGFR2 expression
  • Analytical method of CREB in Ang-2 regulating VEGFR2 expression
  • Analytical method of CREB in Ang-2 regulating VEGFR2 expression

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Embodiment Construction

[0037] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0038] The technical solution of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0039] Such as figure 1 As shown, the analysis method of CREB provided by the embodiments of the present invention in Ang-2 regulation of VEGFR2 expression includes the following steps:

[0040] S101: express the determined protein through the protein expression unit;

[0041] S102: Using the compound to be screened to optimize part of the protein expression unit;

[0042] S103: comparing the protein expression level with the protein expression level in the set control system;

[0043...

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Abstract

The invention belongs to the technical field of the biology, and discloses an analytical method of CREB in Ang-2 regulating VEGFR2 expression. By combining the gene knockout, signal channel inhibitionand like technical measures, the important effect of the CREB in the Ang-2 regulating VEGFR2 expression process is verified, and the determined protein is expressed through a protein expression unit;partial protein expression unit is optimized by adopting a to-be-screened compound; the protein expression quantity is compared with the protein expression quantity in a set reference system, a protein expression result is analyzed to obtain related conclusion for detecting gene toxicity activity. At least partial system is incubated by using the to-be-screened compound, and the protein expression in the system is compared with the protein expression in the reference system, thereby detecting the (front-) gene toxicity activity. The theoretical evidence is provided for the analysis of the CREB in the Ang-2 regulating VEGFR2 expression.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for analyzing CREB in Ang-2 regulating VEGFR2 expression. Background technique [0002] Current, closest prior art: Angiogenesis is essential for solid tumor cell growth and metastasis. Vascular Endothelial Growth Factor (VEGF) promotes tumor angiogenesis and increased blood flow through multiple mechanisms. Vascular Endothelial Growth Factor Receptor (VEGFR), the VEGFR family contains three subtypes, namely VEGFR1 (F1t-1), VEGFR2 (KDR / Flk-1) and VEGFR3 (Flt-4). Among them, VEGFR1 regulates the formation of tumor angiogenesis and affects the number of microvessels; VEGFR2 participates in mediating the proliferation of endothelial cells and induces the formation of tumor blood vessels; VEGFR3 can regulate the formation of tumor lymphatic vessels. Therefore, VEGF and VEGFR, especially VEGFR1 and VEGFR2 are the main targets of anti-angiogenic targeted therapy. [0003] In re...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N33/53G01N33/533G01N33/577G01N33/68
CPCG01N21/6456G01N33/53G01N33/533G01N33/577G01N33/6827
Inventor 邢军超候天勇李志琳程川红许建中
Owner THE FIRST AFFILIATED HOSPITAL OF ARMY MEDICAL UNIV
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