Method for increasing weight of silkworm eggs

A technology of weight and silkworm eggs, applied in the field of improving the weight of silkworm eggs, can solve problems such as lack of research, and achieve the effect of improving the quality of silkworm eggs

Active Publication Date: 2019-10-15
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bombyx mori lipokinetic hormone is a kind of silkworm neuropeptide. The dynamic balance and distribution of energy in the silkworm body is mainly regulated by lipokine...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] According to the sequence pQLTFTSGWGa, an amino acid protected with an α-amino group by a 9-fluorenylmethoxycarbonyl (Fmoc) group is covalently linked to an insoluble solid-phase support (para-alkoxybenzyl alcohol arm) benzyl alcohol type resin); use 90% mass fraction of trifluoroacetic acid (TFA) to take off the Fmoc protecting group on the α-amino group, wash the amino acid--arm--resin with the TFA solution of 90% mass fraction, the next The pre-activated α-amino protected amino acids are connected by a coupling reaction; after the reaction is completed, wash with 90% mass fraction of TFA solution, and then repeat the deprotection and coupling reactions until all amino acids are sequenced from the C-terminus ( carboxyl terminal) to the N terminal (amino terminal) to obtain the target polypeptide; use hydrogen fluoride (HF) to hydrolyze the ester bond between the peptide chain and the solid phase carrier to obtain the silkworm adipokinin A polypeptide synthesis solution...

Embodiment 2

[0035] According to the sequence pQLTFTSGWGa, an amino acid protected with an α-amino group by a 9-fluorenylmethoxycarbonyl (Fmoc) group is covalently linked to an insoluble solid-phase support (para-alkoxybenzyl alcohol arm) benzyl alcohol type resin); use 90% mass fraction of trifluoroacetic acid (TFA) to take off the Fmoc protecting group on the α-amino group, wash the amino acid--arm--resin with the TFA solution of 90% mass fraction, the next The pre-activated α-amino protected amino acids are connected by a coupling reaction; after the reaction is completed, wash with 90% mass fraction of TFA solution, and then repeat the deprotection and coupling reactions until all amino acids are sequenced from the C-terminus ( carboxyl terminal) to the N terminal (amino terminal) to obtain the target polypeptide; use hydrogen fluoride (HF) to hydrolyze the ester bond between the peptide chain and the solid phase carrier to obtain the silkworm adipokinin A polypeptide synthesis solution...

Embodiment 3

[0040] According to the sequence pQLTFTSGWGa, an amino acid protected with an α-amino group by a 9-fluorenylmethoxycarbonyl (Fmoc) group is covalently linked to an insoluble solid-phase support (para-alkoxybenzyl alcohol arm) benzyl alcohol type resin); use 90% mass fraction of trifluoroacetic acid (TFA) to take off the Fmoc protecting group on the α-amino group, wash the amino acid--arm--resin with the TFA solution of 90% mass fraction, the next The pre-activated α-amino protected amino acids are connected by a coupling reaction; after the reaction is completed, wash with 90% mass fraction of TFA solution, and then repeat the deprotection and coupling reactions until all amino acids are sequenced from the C-terminus ( carboxyl terminal) to the N terminal (amino terminal) to obtain the target polypeptide; use hydrogen fluoride (HF) to hydrolyze the ester bond between the peptide chain and the solid phase carrier to obtain the silkworm adipokinin A polypeptide synthesis solution...

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PUM

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Abstract

The invention discloses a method for increasing the weight of silkworm eggs. The method for increasing the weight of the silkworm eggs comprises the following steps: according to the sequence pQLTFTSGWGa, synthesizing silkworm adipokinetic hormone A polypeptide from a C-terminal (carboxy terminal) to an N-terminal (amino terminal); detecting the activity of the synthesized silkworm adipokinetic hormone A polypeptide by a luciferase reporter gene method; and degerming a silkworm adipokinetic hormone A active polypeptide solution by a filtering membrane, injecting the abdomens of female silkwormchrysalises 2-3 days after pupating with the silkworm adipokinetic hormone A active polypeptide solution, and allowing normal copulation after eclosion to produce silkworm eggs with increased weight.The method disclosed by the invention improves the weight of the silkworm eggs by adjusting energy distribution of silkworms by the newly discovered active polypeptide, and has a positive effect on studying the energy distribution mechanism of the silkworms, further improving the quality of the silkworm eggs, and the like.

Description

technical field [0001] The invention relates to a synthetic active polypeptide for increasing the weight of silkworm eggs, in particular to a method for increasing the weight of silkworm eggs. Background technique [0002] The silkworm (Bombyx mori) is the largest economic resource insect on the earth today. It absorbs various nutrients from mulberry leaves and supplies them to various tissues and organs in the silkworm through metabolic and physiological processes for growth, development, and reproduction. Nutrients are supplied to the ovary for silkworm egg production. Sericulture originated in China and has a history of more than 5,000 years. my country is the world's largest sericulture country, and its traditional industry has obvious advantages. The large-scale breeding of silkworms is divided into two types: silkworm cocoon production and silkworm egg production. The main purpose of silkworm egg production is to obtain high-quality and high-yield silkworm eggs, which...

Claims

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Application Information

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IPC IPC(8): C07K14/435C07K1/06C07K1/04C12Q1/66A01K67/04
CPCC07K14/43586C12Q1/66A01K67/04
Inventor 时连根沈张飞屠振力
Owner ZHEJIANG UNIV
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