Collision-induced dissociation pool for ion cascade mass spectrometry and application method thereof

Abstract

The invention discloses an ion collision-induced dissociation pool for ion cascade mass spectrometry. The pool is characterized by comprising a multi-pole electrode system symmetrically distributed about a symmetry central axis of a cascade mass spectrometry electrode system, covering electrodes symmetrically disposed at periphery of multiple poles about the central symmetry axis, and a working power source, wherein the area of a cross section of the covering electrodes along the ion inlet-to-outlet direction of the ion collision-induced dissociation pool gradually increases, the working powersource is used for providing an operating voltage to the multiple poles and the covering electrodes to provide a gradient electric field to make ions move from an entrance of the collision-induced dissociation pool to an outlet. The pool is advantaged in that a potential gradient along the symmetry central axis of the cascade mass spectrum can be generated, fragment ions are accelerated to pass through the ion collision-induced dissociation pool, ion collision energy is enhanced, ion dissociation efficiency is improved, and the time that fragments pass through the ion collision-induced dissociation pool is shortened.

Description

technical field [0001] The invention relates to the technical field of mass spectrometry, in particular to a collision-induced dissociation cell for ion tandem mass spectrometry and a method for using the same. Background technique [0002] Tandem mass spectrometry is one of the common methods to obtain the composition and chemical structure of molecular ions. When quadrupole mass spectrometry is used for tandem mass spectrometry analysis, fast-moving ions are often collided with buffer gas molecules, such as helium and argon, in the ion collision-induced dissociation (CID) cell (induced collision ) to cause ion collision dissociation (induced collision), this pool can also bind the fragmented ion products produced by the collision and transfer them from the collision cell to the downstream mass analyzer for mass analysis of the fragmented ion products. [0003] In tandem quadrupole mass spectrometry, mass-selected precursor ions undergo ion fragmentation in the ion collisi...

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Problems solved by technology

[0007] like figure 1 As shown, the simple CID cell Q2 above is based on a standard quadrupole in RF-only mode, but in this type of CID cell, the ions are not affected by the axial electric field after entering Q2, and the disadvantage is that the remaining parent ions and fragment ions move slowly, making further dissociation difficult to occur, and at the same time, the removal time of its fragmented products is longer

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