sgRNA for up-regulating non-coding RNA expression in human DLK1-DIO3 imprinted domain, recombinant plasmid and cell line
A DLK1-DIO3, recombinant plasmid technology, applied in the field of special sgRNA primer sequences
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
specific Embodiment
[0027] Bladder cancer UM-UC-3 cells in good growth state were cultured. The UM-UC-3 cells were plated one day before virus infection. On the day of infection, dCAS9-VP64 lentiviral particles were added to the groups designed for the experiment to infect the target cells. Puromycine screening was performed 3 days after infection, followed by infection with sgRNA (DLK1-DIO3) lentivirus, G418 screening, and cell collection for detection or related functional studies.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com