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A kind of hydrogel vascular microstent and preparation method thereof

A hydrogel and blood vessel technology, applied in the field of microvascular stents, can solve the problems of low survival rate and differentiation rate, cumbersome process, difficult precision, etc., and achieve high precision and controllability, huge development prospects, and good biocompatibility. Effect

Active Publication Date: 2022-02-01
SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Abstract
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Problems solved by technology

However, the effect is often unsatisfactory, and the following problems may also exist in actual use: 1) the transplanted cells lack normal tissue structure in the local area, and the survival rate and differentiation rate are low; 2) the cytokines are unevenly distributed locally and easily destroyed Normal tissue structure; 3) The release and clearance of drugs are difficult to predict and control; 4) It takes at least 7 days for microvessels to mature from neogenesis, and the reconstruction of microcirculation takes a certain amount of time
[0004] However, the accuracy of the existing method is difficult to make the diameter of the tube less than 600 μm, and the structure cannot completely simulate the hollow tube. 20-200μm diameter) are difficult to simulate remodeling by existing means; at the same time, the process is cumbersome and the controllability is not good, thus limiting the exploration of the early stage of microvascular regeneration

Method used

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  • A kind of hydrogel vascular microstent and preparation method thereof
  • A kind of hydrogel vascular microstent and preparation method thereof
  • A kind of hydrogel vascular microstent and preparation method thereof

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preparation example Construction

[0032] The invention provides a method for preparing a hydrogel vascular microstent, comprising the following steps:

[0033] a) 3D printing the mixed solution of GelMA hydrogel and photoinitiator to obtain GelMA hydrogel sheet;

[0034] b) The GelMA hydrogel sheet obtained in step a) is self-crimped in imitation of apple peel to obtain a hydrogel vascular microstent.

[0035] In the present invention, firstly, the mixed solution of GelMA hydrogel and photoinitiator is 3D printed to obtain the GelMA hydrogel sheet. In the present invention, the GelMA hydrogel can be commercially available or self-made in the laboratory. In a preferred embodiment of the present invention, the GelMA hydrogel is a laboratory product. In the present invention, the preparation method of the GelMA hydrogel is preferably specifically:

[0036] Dissolve gelatin in PBS, heat and stir continuously at 50°C to 90°C, and add methacrylic anhydride to react at the same time; after terminating the reaction...

Embodiment 1

[0075] (1) Use 15wt% gel and 1wt% photoinitiator (Irgacure 2959, Sigma-Aldrich) to mix and use to obtain a GelMA-Irgacure 2959 solution, and print a rectangular shape on a glass slide with the help of a 3D printer (CELLINK AB, Sweden). GelMA hydrogel sheet (size 30mm×2mm×0.20mm);

[0076] The parameters of the 3D printer are: the pneumatic printing nozzle is 200 μm, the temperature of the printing head is 20° C., the temperature of the glass slide on the printing platform is 10° C., and the printing speed is 10 mm / s.

[0077] (2) After cross-linking the GelMA hydrogel sheet obtained in step (1) with 365nm ultraviolet light (XL-1000A) for 2min, then completely dry it at 25°C; then immerse the glass slide in water, and the GelMA hydrogel The sheet was peeled off from the glass slide, and the self-rolling phenomenon could be observed; finally, the successfully curled gel tube was immersed in water for 2 hours to remove excess photoinitiator, and then freeze-dried to obtain the hy...

Embodiment 2

[0082] (1) Use 10wt% gel and 1wt% photoinitiator (Irgacure 2959, Sigma-Aldrich) to mix and use to obtain a GelMA-Irgacure 2959 solution, and print a rectangular shape on a glass slide with the help of a 3D printer (CELLINK AB, Sweden). GelMA hydrogel sheet (size 30mm×2mm×0.20mm);

[0083] The parameters of the 3D printer are: the pneumatic printing nozzle is 200 μm, the temperature of the printing head is 20° C., the temperature of the glass slide on the printing platform is 10° C., and the printing speed is 10 mm / s.

[0084] (2) After cross-linking the GelMA hydrogel sheets obtained in step (1) with 365nm ultraviolet light (XL-1000A) for 0.5min, then completely dry them at 25°C; The film was peeled off from the glass slide, and the self-rolling phenomenon could be observed; finally, the successfully curled gel tube was immersed in water for 2 hours to remove excess photoinitiator, and then freeze-dried to obtain the hydrogel vascular microstent.

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Abstract

The invention provides a hydrogel vascular microstent and a preparation method thereof. The preparation method comprises the following steps: a) 3D printing a mixed solution of GelMA hydrogel and a photoinitiator to obtain a GelMA hydrogel sheet; b) Self-crimping the GelMA hydrogel sheet obtained in step a) to obtain a hydrogel vascular microstent. Compared with the prior art, the preparation method provided by the present invention constructs hydrogel vascular microstents by imitating the self-curling of apple peel, which is different from the previous 3D printing, and can construct finer micro-hollow tubes (below 200 μm), Moreover, the present invention can first construct gel sheets of different shapes and sizes, and then obtain gel tubes of corresponding forms, which has higher precision and controllability; and, the hydrogel vascular microstent obtained by the preparation method provided by the present invention It has good cell affinity, good biocompatibility, and rich forms, which can basically meet the bionic needs of all forms of vascular stents, and has great development prospects.

Description

technical field [0001] The invention relates to the technical field of microvascular stents, in particular to a hydrogel vascular microstent and a preparation method thereof. Background technique [0002] The complex vascular network is crucial to the survival and repair of tissues. Through the large blood vessels to the micro vascular network, blood continuously circulates and transports nutrients and metabolites; otherwise, it will lead to some difficult problems such as skin flap necrosis, coronary heart disease, and osteonecrosis . During the process of vascularization, various angiogenic cells such as endothelial cells migrate to form primary capillaries, which further develop into arterioles and venules. Based on this, many studies are dedicated to improving tissue blood supply, accelerating tissue regeneration, and reducing inflammatory responses through functional cells (e.g., mesenchymalstem cells), cytokines (e.g., VEGF), and drugs (e.g., aminoguanidine). However...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L31/04A61L31/14A61F2/07
CPCA61L31/045A61L31/145A61L31/14A61F2/07A61F2002/821
Inventor 孙晓明张柳成刘芷默程丽英张路毛曦媛陈露崔文国张余光
Owner SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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