Interfering RNA (Ribonucleic Acid) for inhibiting expression of echinococcus granulosus protoscolex DNA (Deoxyribonucleic Acid) oxidative damage repairing gene and application of interfering RNA

Abstract

The invention relates to the technical field of bio-medicine engineering and discloses an interfering RNA (Ribonucleic Acid) for inhibiting expression of an echinococcus granulosus protoscolex DNA (Deoxyribonucleic Acid) oxidative damage repairing gene and application of the interfering RNA. The interfering RNA for inhibiting expression of the echinococcus granulosus protoscolex DNA oxidative damage repairing gene has target sequences of forward CCGUGUUCGUAAGCAGCUUTT and backward AAGCUGCUUACGAACACGGTT. The invention discloses the target sequences of the interfering RNA for inhibiting expression of the echinococcus granulosus protoscolex DNA oxidative damage repairing gene for a first time, and also discloses application of the interfering RNA in preparing cystic echinococcosis medicines for the first time. By inhibiting expression of the EgRPS9 gene by using the interfering RNA, growth of echinococcus granulosus protoscolex is inhibited and killed by affecting the repairing function of DNA oxidation damage of the echinococcus granulosus protoscolex, so that cystic echinococcosis can be effectively treated.

Description

technical field [0001] The invention relates to the technical field of biomedical engineering, and relates to an interference RNA for inhibiting the expression of the DNA oxidative damage repair gene of the echinococcus granulosus protoscole and its application. Background technique [0002] Cystic echinococcosis (CE), also known as cystic echinococcosis, is a serious zoonotic parasitism caused by the larvae of Echinococcus granulosus in humans or host animals. Insect diseases, listed by WHO as one of Neglected Tropical Diseases (NTDs), are global public health problems. Domestic dogs are the main source of infection and final host (Echinococcus disease). When domestic dogs excrete mature proglottis and a large number of eggs, they will pollute grassland, water sources, home environment or attach to their fur. Infected by worm eggs. [0003] Echinococcus granulosus is more common in countries and regions with developed animal husbandry. The main endemic countries are China...

AI Technical Summary

Problems solved by technology

However, due to the complexity of the disease, complete excision of the exocyst cannot be achieved, resulting in exocysts containing active Echinococcus granulosus protoscolmets still remaining in the patient, and the postoperative recurrence rate is high, so it is only suitable for cystic resection The number of patients with echinococcosis is small and early stage, but the damage to the patients is serious and the cost of treatment is high. To improve the quality of life of recurrent, multiple, and advanced patients, drug therapy is irreplaceable

[0005] At present, only benzimidazoles are the only drugs that have been approved for the clinical treatment of echinococcosis. Clinical studies have found that although these drugs can prolong the life of patients and relieve clinical symptoms, they cannot completely cure the disease.

This type of drug has disadvantages such as poor intestinal absorption rate, low blood drug concentration and liver drug concentration, resulting in the effective drug concentration acting on the lesion site far below the proper therapeutic concentration, which weakens the therapeutic effect

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