A kind of compound passivator of cadmium polluted soil and its application
A technology of soil passivation and passivating agent, applied in the field of soil remediation, can solve the problem that the passivating agent cannot achieve the effect of soil remediation, etc., and achieve the effects of being less prone to diseases and insect pests, promoting plant growth, and reducing bioavailability
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[0044] The soil passivation agent is compounded with carrier passivation materials (calcium citrate-iron-based materials and mercapto swelling materials) to prepare a soil composite passivation agent with nutrient slow-release effect. Concrete preparation method comprises the following steps:
[0045] 1. Preparation of calcium citrate iron-based materials
[0046] (1) Mix ferrous sulfate heptahydrate with anhydrous sodium carbonate and citric acid in a molar ratio of 1:1:1, adjust the pH to 7.0-8.0 with sodium hydroxide or calcium oxide, and obtain substance A.
[0047] (2) Mix citric acid and calcium oxide at a molar ratio of 2:3, adjust the pH to 7.0-8.0 with sodium hydroxide, wash with water, and filter to obtain white precipitate B.
[0048] (3) The substance A in the step (1) and the precipitate B in the step (2) are mixed in a mass ratio of 1:1 to 4, uniformly mixed, and dried to obtain a calcium citrate-iron-based material.
[0049] 2. Preparation of mercapto swelling...
Embodiment 1
[0063] Embodiment one: the preparation of soil passivation agent
[0064] The Pseudomonas is inoculated into the culture medium, and the culture medium composition is: KNO 3 1.0g, KH 2 PO 4 1.0g, MgSO 4 ·7H 2 O 1.0g, FeCl 3 ·6H 2 O 0.05g, CaCl 2 2H 2 O 0.2g, trisodium citrate 5.13g, distilled water 1000ml.
[0065] After culturing on a shaker at 30° C. and 150 rpm for 24 hours, the strains were activated. Transfer to a new medium according to the inoculum amount of 1%, and culture at 30° C. for another 24 hours to obtain a seed liquid.
[0066] The seed solution is inserted into a fermenter (containing a new medium) according to the inoculum amount of 1% to expand the culture, cultivated at 28° C. for 16 hours, and the resulting bacterial suspension is collected, which is the soil passivation agent.
Embodiment 2
[0067] Embodiment 2: Application of soil passivation agent (showing the effect of soil passivation agent improving cadmium-polluted water quality hydroponic wheat)
[0068] Greenhouse hydroponic experiments were carried out using the culture medium added with heavy metal Cd (5mg / L) as the substrate. After the wheat seeds were germinated in clear water and turned white, the seeds with normal germination and no significant difference between groups were selected and placed in the germination basket with colonized cotton, then the colonized cotton was soaked in the culture solution containing heavy metal Cd, and inserted into the 1 The soil passivating agent prepared by the embodiment one of %, as experimental group; Simultaneously with not adding soil passivating agent as control group, each experimental group establishes 4 repetitions; Simultaneously with the clear water containing the Cd of 5mg / L, as blank Group. The cultivation conditions in the greenhouse were 12 hours duri...
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