Method for preparing beta-glucan in highland barley vinasse
A technology of dextran and highland barley wine, which is applied to medical preparations containing active ingredients, pharmaceutical formulas, food science, etc., can solve the problems of surrounding environmental pollution, corruption and deterioration, and achieve high-value comprehensive utilization and low cost , The method is simple and easy to implement
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[0053] The preparation method of β-glucan in highland barley distiller's grains comprises the following steps:
[0054] 1) drying the highland barley distiller's grains to a moisture content of 12.5%, removing impurities (gravel, etc.), crushing, and passing through a 30-mesh sieve to obtain highland barley distiller's grains powder;
[0055] 2) Weigh 300g of the above highland barley distiller's grains powder, add 3000ml of 80% ethanol, mix well, boil and reflux for 2 hours, filter, take the filter residue and dry at 40°C for 12 hours;
[0056] 3) Weigh 120g of distiller's grain filter residue dried in step 2), add 1200mL of water, stir evenly, adjust the pH to 8.0 with sodium hydroxide solution, heat and stir at 70°C for 4h, centrifuge at 6000r / min for 5min, remove the precipitate, and obtain β - Dextran crude extract.
[0057] 4) High temperature α-amylase enzymatic hydrolysis:
[0058] Adjust the pH of the crude β-glucan extract in step 3) to 6.0, add 2.4 g of high-tempera...
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