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Preparation method of microbial agent for plant disease prevention and cure

A microbial agent and plant disease technology, applied in the field of microbial agent preparation, can solve the problems of restricting the sustainable development of agriculture, threatening human health, residual environment, etc., to increase the ability of plants to resist adversity, enhance root growth and nutrient absorption , the effect of inhibiting growth and reproduction

Pending Publication Date: 2019-08-30
HANGZHOU LANDSCAPE ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, plant diseases are mainly prevented and controlled by chemical pesticides, but the long-term use of chemical pesticides in large quantities has brought about a series of problems such as pathogen resistance, pesticide residues in agricultural products, and environmental pollution, which seriously restricts the sustainable development of agriculture and threatens human health

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A preparation method of a microbial inoculant for preventing and controlling plant diseases, the steps are as follows:

[0026] 1) The preparation includes the following raw materials in parts by weight: 10 parts of Paecilomyces lilacinus secondary bacterial liquid, 3 parts of Bacillus licheniformis, 3 parts of Sphingobacterium, 2 parts of Gram-positive actinomycetes, and Bacillus cereus 2 parts, 6 parts of nitrogen-fixing bacteria, 2 parts of Bacillus megaterium, 2 parts of photosynthetic bacteria, 8 parts of amino acids, 1 part of humic acid, 1 part of monosaccharide, 3 parts of chitin, and 4 parts of modified diatomaceous earth.

[0027] 2) Bacillus licheniformis and Sphingobacterium are made into Bacillus licheniformis-Sphingobacterium mixed fermentation broth;

[0028] 3) making Gram-positive actinomycetes-Bacillus cereus mixed fermentation broth with Gram-positive actinomycetes and Bacillus cereus;

[0029] 4) Mixing the secondary bacterial liquid of Paecilomyces...

Embodiment 2

[0038] A preparation method of a microbial inoculant for preventing and controlling plant diseases, the steps are as follows:

[0039] 1) The preparation includes the following raw materials in parts by weight: 12 parts of Paecilomyces lilacinus secondary bacteria liquid, 7 parts of Bacillus licheniformis-Sphingobacterium mixed fermentation broth, mixed Gram-positive actinomycetes-Bacillus cereus 5 parts of fermentation broth, 6.5 parts of nitrogen-fixing bacteria, 3 parts of Bacillus megaterium, 2.5 parts of photosynthetic bacteria, 9 parts of amino acids, 1.5 parts of humic acid, 1.5 parts of monosaccharide, 4 parts of chitin, and 4.5 parts of modified diatomaceous earth.

[0040] 2) Bacillus licheniformis and Sphingobacterium are made into Bacillus licheniformis-Sphingobacterium mixed fermentation broth;

[0041] 3) making Gram-positive actinomycetes-Bacillus cereus mixed fermentation broth with Gram-positive actinomycetes and Bacillus cereus;

[0042]4) Mixing the seconda...

Embodiment 3

[0051] A preparation method of a microbial inoculant for preventing and controlling plant diseases, the steps are as follows:

[0052] 1) The preparation includes the following raw materials in parts by weight: 15 parts of Paecilomyces lilacinus secondary bacterial liquid, 8 parts of Bacillus licheniformis-Sphingobacterium mixed fermentation broth, mixed Gram-positive actinomycetes-Bacillus cereus 6 parts of fermentation broth, 7 parts of nitrogen-fixing bacteria, 4 parts of Bacillus megaterium, 3 parts of photosynthetic bacteria, 10 parts of amino acids, 2 parts of humic acid, 2 parts of monosaccharide, 5 parts of chitin, and 5 parts of modified diatomite;

[0053] 2) Bacillus licheniformis and Sphingobacterium are made into Bacillus licheniformis-Sphingobacterium mixed fermentation broth;

[0054] 3) making Gram-positive actinomycetes-Bacillus cereus mixed fermentation broth with Gram-positive actinomycetes and Bacillus cereus;

[0055] 4) Mixing the secondary bacterial liq...

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Abstract

The invention discloses a preparation method of a microbial agent for plant disease prevention and cure, and relates to the technical field of microbial prevention and cure of plant diseases. The steps are as below: (1) preparing the following raw materials: Paecilomyces lilacinus secondary bacterial fluid, Bacillus licheniformis-Sphingobacterium mixed fermentation fluid, Gram-positive actinomycetes-Bacillus cereus mixed fermentation fluid, nitrogen-fixing bacteria, Bacillus megaterium, photosynthetic bacteria, amino acids, humic acids, monosaccharides, chitin and modified diatomaceous earth;(2) preparing the Bacillus licheniformis-Sphingobacterium mixed fermentation fluid; (3) preparing the Gram-positive actinomycetes-Bacillus cereus mixed fermentation fluid; (4) preparing a mixed bacterial fluid A; and (5) adding the remaining raw materials to the mixed bacterial fluid A to obtain the microbial agent for plant disease prevention and cure. According to the present invention, the microbial agent contains a plurality of beneficial bacteria species, is effective for eliminating root-knot nematodes and eggs in soil by inhibiting bacteria with bacteria and by killing pests with bacteria, and is effective for inhibiting the growth and reproduction of soil-borne pathogenic bacteria.

Description

technical field [0001] The invention relates to the technical field of microbial prevention and control of plant diseases, in particular to a preparation method of a microbial agent for the prevention and treatment of plant diseases. Background technique [0002] Microorganisms include: bacteria, viruses, fungi, and some small protozoa, microscopic algae, etc., which are small in size and closely related to human beings. Covers many types of beneficial and harmful, widely involved in food, medicine, industry and agriculture, environmental protection, sports and many other fields. In Chinese textbooks, microorganisms are divided into the following eight categories: bacteria, viruses, fungi, actinomycetes, rickettsia, mycoplasma, chlamydia, and spirochetes. Some microorganisms are visible to the naked eye, such as mushrooms belonging to fungi, ganoderma lucidum, and shiitake mushrooms. There are also microorganisms, which are a class of "non-cellular organisms" composed of a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/20A01N63/00A01N63/04A01P5/00C12R1/79C12R1/10C12R1/085C12R1/065C12R1/11C12R1/01
CPCC12N1/14C12N1/20A01N63/00A01N63/30
Inventor 徐钟杰宁凯檀会珍郭涌梁董生赵淑芳
Owner HANGZHOU LANDSCAPE ENG
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