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Hydrogel preparation based on quantum dots and gold nanorods as well as preparation method and application of hydrogel preparation

A technology of gold nanorods and quantum dots, which is applied in the field of hydrogel preparations based on quantum dots and gold nanorods and its preparation, and can solve problems such as recurrence, large economic burden on patients, and inability to detect

Inactive Publication Date: 2019-08-02
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And despite the continuous improvement of surgical techniques over the years, it is inevitable that there will be residual cancer cells after surgery, so postoperative recurrence often occurs
However, postoperative in situ recurrence is not visible in the early stage, and existing methods such as B-ultrasound and CT cannot detect it
At present, there are few products clinically used to monitor orthotopic recurrence after tumor surgery, and the price is expensive, which brings a great economic burden to patients

Method used

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  • Hydrogel preparation based on quantum dots and gold nanorods as well as preparation method and application of hydrogel preparation
  • Hydrogel preparation based on quantum dots and gold nanorods as well as preparation method and application of hydrogel preparation
  • Hydrogel preparation based on quantum dots and gold nanorods as well as preparation method and application of hydrogel preparation

Examples

Experimental program
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Effect test

Embodiment 1

[0030] (1) Preparation of carcinoembryonic antigen aptamer-modified quantum dots: Use double distilled water to prepare carboxyl modified quantum dots into a solution with a concentration of 1.5 mg / ml, and add 20 μl of amino groups at a concentration of 50 μmol / l The modified carcinoembryonic antigen aptamer solution was stirred magnetically for 12 hours, and the precipitate was collected by centrifugation at 12,000 rpm for 10 minutes. The precipitate was resuspended in double-distilled water and quantified to 1 mg / ml to obtain the carcinoembryonic antigen aptamer modified quantum dots .

[0031] (2) Preparation of gold nanorods modified by carcinoembryonic antigen aptamer: Prepare gold nanorods with double distilled water to a solution with a concentration of 1 mg / ml, and add 20 microliters of aptamer with a concentration of 50 micromoles per liter. After stirring for 24 hours, the precipitate was collected by centrifugation at 12,000 rpm for 30 minutes, and the precipitate was ...

Embodiment 2

[0034] (1) Preparation of carcinoembryonic antigen aptamer-modified quantum dots: use double distilled water to prepare carboxyl modified quantum dots into a solution with a concentration of 1.2 mg / ml, and add 15 μl of amino groups at a concentration of 50 μmol / l The modified carcinoembryonic antigen aptamer solution was stirred magnetically for 8 hours, and the precipitate was collected by centrifugation at 11,000 rpm for 8 minutes. The precipitate was resuspended in double-distilled water and quantified to 0.8 mg / ml to obtain the carcinoembryonic antigen aptamer modified quantum dots . Pipette 1 ml of QDs modified with carcinoembryonic antigen aptamer and add them to a glass dish. Use a fluorescence spectrometer to detect the fluorescence intensity at an excitation wavelength of 450 nm. The result is as follows figure 1 Shown.

[0035] (2) Preparation of gold nanorods modified by carcinoembryonic antigen aptamer: Prepare gold nanorods with double distilled water into a solution...

Embodiment 3

[0038] (1) Preparation of carcinoembryonic antigen aptamer-modified quantum dots: prepare the carboxyl modified quantum dots with double distilled water into a solution with a concentration of 1 mg / ml, and add 10 microliters of amino groups at a concentration of 50 micromoles per liter The modified carcinoembryonic antigen aptamer solution was magnetically stirred for 4 hours, and the precipitate was collected by centrifugation at 10,000 rpm for 5 minutes. The precipitate was resuspended in double-distilled water and quantified to 0.5 mg / ml to obtain the carcinoembryonic antigen aptamer modified quantum dots .

[0039] (2) Preparation of gold nanorods modified by carcinoembryonic antigen aptamer: Prepare gold nanorods with double distilled water to a solution with a concentration of 0.5 mg / ml, and add 10 microliters of aptamer with a concentration of 50 micromoles per liter. The body solution was stirred for 12 hours and centrifuged at 10,000 rpm for 15 minutes to collect the pre...

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Abstract

The invention provides a preparation method of a hydrogel preparation based on quantum dots and gold nanorods. The preparation method comprises the following steps of firstly, combining the quantum dots with a carcinoembryonic antigen aptamer; secondly, combining the gold nanorods with the carcinoembryonic antigen aptamer; thirdly, mixing the quantum dots modified by the carcinoembryonic antigen aptamer, the gold nanorods modified by the carcinoembryonic antigen aptamer and a hydrogel solution; and finally, obtaining the hydrogel preparation based on the quantum dots and the gold nanorods. Theprepared hydrogel preparation can be used for detecting a carcinoembryonic antigen, and when the hydrogel preparation is co-incubated with the carcinoembryonic antigen whose concentration of 1-6 microliters is 1 mg / ml, the light quenching efficiency is 12-24%.

Description

Technical field [0001] The invention relates to the technical field of biomedicine, in particular to a hydrogel preparation based on quantum dots and gold nanorods, and a preparation method and application thereof. Background technique [0002] At present, surgery, chemotherapy and radiotherapy are the main methods of cancer treatment. Early-stage cancer can be cured by surgical resection. However, according to statistics, about 90% of cancer patients eventually die because of tumor recurrence or cancer metastasis. This is mainly due to the fact that some tumor cells are already in the blood or lymph vessels before the tumor is removed. Moreover, despite the continuous improvement of surgical techniques over the years, residual cancer cells are inevitable after surgery, so postoperative recurrence often occurs. However, the early postoperative recurrence in situ is not visible, and the existing methods such as B-ultrasound and CT cannot be detected. At present, there are few p...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N21/64G01N33/533G01N33/58
CPCG01N21/6428G01N33/533G01N33/57484G01N33/588G01N2021/6432G01N2800/7028
Inventor 常津张英英王汉杰田雨王天歌
Owner TIANJIN UNIV
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