A method for removing prunoside from passion fruit peel crude extract
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A technology of prunoside and passion fruit, which is applied in the field of extraction of active ingredients in plants, can solve problems such as potential safety hazards, and achieve low cost effects
Active Publication Date: 2021-09-24
GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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[0006] It can be seen that if the anthocyanin extract of passion fruit peel containing prunoside is directly applied to food, medicine and other fields, there will be certain potential safety hazards
Method used
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Embodiment 1
[0025] 1) Take 500g of passion fruit peel (20 mesh), add 10L of ethanol solution with pH=2.5 and concentration of 70v / v% to extract at room temperature for 60min, filter, repeat the above extraction operation twice for the filter residue, combine the filtrate, and recover the obtained extract Ethanol, obtains the crude extract of passion fruit peel;
[0026] 2) Put the crude extract of passion fruit peel on a macroporous resin column (D101) for chromatography, first wash the column with 5BV of pure water with pH=2.5 to remove impurities such as sugar and protein; / v% ethanol solution for elution, collect the eluate with obvious red color, and concentrate in vacuo to obtain concentrated anthocyanin solution;
[0027] 3) Pass the anthocyanin concentrate through a Sephadex LH-20 column, elute with a methanol solution with a pH of 2.5 and a concentration of 10v / v%, and use a UV-visible spectrophotometer and TLC Analyze and monitor the elution process, collect the eluate whose abs...
Embodiment 2
[0037] Repeat Example 1, the difference is:
[0038] In step 3), elute with a methanol solution with a pH of 2.5 and a concentration of 20v / v%.
[0039] Finally, 489 mg of the passion fruit peel anthocyanin extract was obtained.
Embodiment 3
[0041] Repeat Example 1, the difference is:
[0042] In step 2), elute with ethanol solution with pH=2.5 and concentration of 30v / v%;
[0043] In step 3), elute with a methanol solution with a pH of 2.5 and a concentration of 20v / v%.
[0044] Finally, 432 mg of the passion fruit peel anthocyanin extract was obtained.
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Abstract
The invention discloses a method for removing prunoside from the crude extract of passion fruit peel, comprising the following steps: 1) obtaining the crude extract of passion fruit peel; 2) putting the crude extract of passion fruit peel on a macroporous resin column Chromatography, elution and concentration to obtain concentrated anthocyanins; 3) passing the concentrated anthocyanins through a Sephadex resin column, washing with a methanol solution with a pH of 1-3 and a concentration of 10-20v / v% Take off, collect the fractions containing the target substance, concentrate, and dry to obtain the anthocyanin extract of passion fruit peel. Compared with the prior art, the present invention creatively selects methanol solution with pH=1-3 and a concentration of 10-20v / v% as the eluent to elute when passing through the Sephadex resin column, effectively improving the The anthocyanin content in the obtained passion fruit peel anthocyanin extract is also well removed prunin, so that the obtained passion fruit peel anthocyanin extract can be directly applied to the fields of food and medicine.
Description
technical field [0001] The invention relates to a method for extracting effective components in plants, in particular to a method for removing prunoside from the crude extract of passion fruit peel. Background technique [0002] Passion fruit (Passiflora edulia Sims), also known as egg fruit, is a herbaceous vine of Passiflora genus Passiflora. The main production areas in my country are Guangdong, Guangxi, Taiwan, Fujian and other provinces. Passion fruit juice is rich in nutrients and has a strong and unique fragrance. It has the reputation of "king of juice". Passion fruit is currently mainly used to produce juice, and a very small part is eaten as fresh fruit. In the production of a large amount of passion fruit juice, passion fruit peel is discarded as waste, which not only causes a great waste of resources, but also imposes a burden on the environment. [0003] Anthocyanins are a class of compounds widely present in the flowers, fruits, stems, leaves and other organs...
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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/065C07H1/08
CPCC07H1/08C07H17/065
Inventor 符毓夏潘争红宁德生李连春
Owner GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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