Method for detecting acetamiprid based on polypyrrole ferric oxide and fluorescent material

A technology for polypyrrole tetroxide and ferroferric oxide magnetism, which is applied in the field of chemical analysis, can solve problems such as inability to achieve specific identification, and achieve the effects of simple method, good selectivity and high reaction efficiency

Active Publication Date: 2021-06-25
东莞海关综合技术中心 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method cannot achieve specific recognition of acetamiprid, which has certain limitations

Method used

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  • Method for detecting acetamiprid based on polypyrrole ferric oxide and fluorescent material
  • Method for detecting acetamiprid based on polypyrrole ferric oxide and fluorescent material
  • Method for detecting acetamiprid based on polypyrrole ferric oxide and fluorescent material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] 1. Synthesis of ferroferric oxide polypyrrole magnetic material

[0058] 1. Synthesis of Ferroferrofluid

[0059] Weigh 2.4g of NaOH and 2.0g of polyethylene glycol, add 100ml of deionized water, stir to dissolve, then pour into the reaction kettle, and store with nitrogen gas.

[0060] Weigh 2.7g FeCl 3 .6H 2 O, 1.9g FeCl 2 .4H 2 O, add 100ml deionized water, stir to dissolve, and pour into a separatory funnel.

[0061] In a microwave sonicator, the reactor was sonicated for 1 hour while FeCl 3 .6H 2 O and FeCl 2 .4H 2 The O mixture was added to the reaction kettle within 20 minutes.

[0062] After the reaction is complete, pour the solution into a beaker and let it stand, pour off the supernatant, wash the remaining liquid with ultrapure water repeatedly, and filter and dry it for later use.

[0063] 2. Fe 3 o 4 @Ppy microsphere synthesis

[0064] Weigh 0.14g of sodium dodecylsulfonate, add 85ml of deionized water, pour into the reaction kettle and sonica...

Embodiment 2

[0067] The effect of TTAPE concentration on the fluorescence intensity was investigated. Take 200ul of TTAPE solution and add it into a 5ml volumetric flask. Add 1 μM acetamiprid aptamer solution to constant volume, and prepare a 0-2000 nM acetamiprid aptamer solution. Shake at 30°C for 30 minutes, then detect fluorescence.

[0068] Such as figure 2 As shown, the results showed that as the concentration of acetamiprid aptamer increased, the fluorescence intensity gradually increased due to the combination of TTAPE and DNA. When the intensity reaches 1000nM, the fluorescence intensity reaches a stable value.

Embodiment 3

[0070] Fe 3 o 4 The usage of @Ppy. Take 200 μL of AIE solution (100 μM) and add it to a 5ml volumetric flask. Add acetamiprid aptamer solution (1 μM) to constant volume. Weigh 0-40mgFe 3 o 4 @Ppy Yu solution,

[0071] Shake for 30 minutes, observe the law of fluorescence quenching.

[0072] The result is as image 3 As shown, it was found that about 15 mg of polypyrrole had an obvious fluorescence quenching effect, so 15 mg of polypyrrole was selected as the final dosage.

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Abstract

The invention provides a method for detecting acetamiprid based on polypyrrole ferric oxide and a fluorescent material. The specific steps are as follows: 1) take the AIE fluorescent material solution and mix the acetamiprid aptamer solution and shake it, and detect the fluorescence intensity F of the solution. 0 ; 2) adding polypyrrole ferric oxide to the mixed solution obtained in step 1), and the fluorescence disappears after shaking; 3) adding the sample solution to be tested into the mixed solution obtained in step 2) and shaking, and detecting the fluorescence intensity F of the solution; 4) according to the standard The fluorescence response curve is used to obtain the concentration c of acetamiprid in the sample solution to be tested. The detection method of the invention has high sensitivity, good selectivity, specific recognition function and no interference from other substances.

Description

technical field [0001] The invention relates to the technical field of chemical analysis, in particular to a method for detecting acetamiprid based on polypyrrole ferric oxide and a fluorescent material, especially a method for detecting acetamiprid by using an AIE nucleic acid probe and polypyrrole ferric oxide. Background technique [0002] In 2001, Academician Tang Benzhong’s research group found that the molecule had no fluorescence emission in the solution state when studying the hexaphenylsilole (HPS) molecule. When the molecule was in an aggregated state or in a solid state, its fluorescence intensity was significantly enhanced. This phenomenon is called It is aggregation-induced emission (AIE). This original concept has led the upsurge of research all over the world since it was proposed, and the team of Academician Tang Benzhong also won the first prize of the National Natural Science Award in 2017. The reason why AIE materials can cause extensive theoretical resea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6402G01N21/6486
Inventor 黄雪琳钟帮奇温健昌郭宗宁焦哲张鹏飞
Owner 东莞海关综合技术中心
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