Method for measuring optimum sampling period of rice stigma

Abstract

The invention discloses a method for measuring the optimum sampling period of a rice stigma. A full bloom glumous flower (when the length of the rice stigma reaches the maximum) serves as a sample tobe sampled, firstly, the unified standard is used for sampling, time nodes are easy to control; and secondly, the error generated due to the fact that the sampling periods of different researchers aredifferent in the sampling process is reduced. Data of phenotypic values of obtained length characters of sampled stigma in the period (when a spikelet glumous flower blooms) are accurate, repeatability is high, obtained results can be used for excavating favorable genes of long stigmas, and finely locating, cloning and function-analyzing the stigma length characters; and meanwhile, a rice sterileline is improved by using the long stigma gene excavated by the sampling method, the stigma length of the rice sterile line can be increased, thus the rice stigma exposing rate is improved, and the hybrid seed yield is increased.

Description

technical field [0001] The invention belongs to the field of investigation methods for rice outcrossing-related traits, and in particular relates to a method for measuring the optimal sampling period of rice stigma length. Background technique [0002] Rice is the second largest food crop in the world, and more than 50% of the world's population uses rice as the main source of food. As the largest rice producer in the world, my country has a total annual output of more than 200 million tons, and about 700 million people use rice as a staple food. With the continuous growth of population and the decrease of arable land at the rate of millions of acres per year, it is an inevitable choice to greatly increase the output per unit area to ensure food security. Hybrid rice, which began in my country in the 1970s, has an increase of about 15% per unit area compared with conventional rice at the same maturity period, and its commercial planting has greatly improved rice productivit...

AI Technical Summary

Problems solved by technology

[0005] However, there are few research reports on the stigma length of rice at present, which may be caused by the following two reasons: First, the stigma length sampling and storage procedures are cumbersome, and the stigma is easily damaged

In 2012, Chen Lan et al. took 5 spikelets from each panicle to investigate the length of the stigma when the anthers were mature but not scattered. At noon or in the afternoon, investigate the stigma length of 5 florets that open that day. This method may cause errors in the measurement of the stigma length phenotype data due to the expansion of the ovary and the degeneration of the stigma after the rice florets are fertilized in the afternoon.

Second, because there is no uniform measurement standard, Chen Lan et al. (2012) stripped and measured 5 glumes per material under a binocular dissecting microscope; while Wu Jian et al. (2017) also took 5 glumes per material. Flowers are photographed with a camera, and then the length of the stigma is measured with Image J software, the measurement procedure is cumbersome

Therefore, when sampling rice stigma length surveys, the phenotype data of samples at different developmental stages will cause huge errors in the test results

The length of the rice stigma itself is very small (so far, 1.0mm-3.5mm), and any error in any link in the sampling and measurement process will bring great differences to the phenotypic data