Application of 6-amyl-2H-pyran-2-ketone compound in preparing microbial agent for resisting fusarium oxysporum f.sp.cubense 4 physiological race and ustilaginoidea virens
A technology for banana fusarium wilt and rice false smut, applied in the directions of botanical equipment and methods, applications, fungicides, etc., can solve the problems of reducing rice yield, affecting rice quality, causing food safety problems, etc., and achieving a good control effect. Effect
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[0027] Inhibitory effect of 6-pentyl-2H-pyran-2-one compound on Fusariumoxysporum f.sp.cubense race 4 and Ustilagi noideavirens
[0028] 1. Bacterial solution preparation: Prepare 100mL of PDB medium and put it in a 500mL Erlenmeyer shake flask, pick it from the previously activated PDA plate with Fusarium oxysporum f.sp.cubense race 4 and Ustilaginoideavirens pathogenic bacteria Small bacterial blocks were respectively placed in PDB medium, placed in a shaker at 120r / min, and cultured at 28°C for 24h. Then take a small amount of cultured bacterial solution and dilute it to 1×10 with sterile PDB medium. 8 Spores / ml (hemocytometer count).
[0029] 2. Sample solution preparation: the 6-pentyl-2H-pyran-2-one monomer compound to be tested was prepared into a solution with a concentration of 15 mg / mL in methanol for use.
[0030] 3. Specific operation: Take 10 μL of the diluted pathogen spore solution, drop them on the sterilized filter paper sheets of about 1 cm in size, and aft...
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