Protein feed optimized by compound micro-organism solid-state aerobic fermentation and preparation method thereof

A technology of protein feed and aerobic fermentation, applied in biochemical equipment and methods, animal feed, animal feed, etc., can solve the problems of lack, restriction of microbial application, low enzyme production capacity, etc.

Pending Publication Date: 2019-07-19
SOUTHWEST UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the enzyme-producing ability of existing microbial strains is low, which limits the application of microorganisms in the feed industry. At the same time, there is a lack of research on mixed solid-state oxygen consumption of cottonseed meal and rapeseed meal by complex bacteria of Aspergillus oryzae, Aspergillus niger and Bacillus subtilis

Method used

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  • Protein feed optimized by compound micro-organism solid-state aerobic fermentation and preparation method thereof
  • Protein feed optimized by compound micro-organism solid-state aerobic fermentation and preparation method thereof
  • Protein feed optimized by compound micro-organism solid-state aerobic fermentation and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0084] Embodiment 1, the protein feed optimized by compound bacteria solid-state aerobic fermentation

[0085] The protein feed optimized by the solid-state aerobic fermentation of compound bacteria, the weight ratio of each raw material is: 1000 kg of mixed fermentation raw materials, 800 grams of compound bacteria;

[0086] The composition and weight ratio of the mixed fermentation raw materials are: 4 parts of cottonseed meal, 5 parts of rapeseed meal, 2 parts of fresh distiller's grains; the moisture content of the fresh distiller's grains is 58-60%;

[0087] The weight ratio of each raw material in the composite strain is: 4 parts of Aspergillus niger (CICC2139AS3.324), 4 parts of Aspergillus oryzae (CICC2329 Huyao 3.042), and 2 parts of Bacillus subtilis (BNCC188080);

[0088] The number of living bacteria of each raw material in the composite fermentation agent: Aspergillus niger (CICC2139 AS3.324) 1.10×10 10 ~1.15×10 10 cfu / g, Aspergillus oryzae (CICC2329 Shanghai Br...

Embodiment 2

[0090] Embodiment 2, the protein feed optimized by compound bacteria solid-state aerobic fermentation

[0091] The protein feed optimized by compound bacteria solid-state aerobic fermentation, the weight ratio of each raw material is: 1200 kg of mixed fermentation raw materials, 800 grams of compound bacteria;

[0092] The composition and weight ratio of the mixed fermentation raw materials are: 5 parts of cottonseed meal, 4 parts of rapeseed meal, 2 parts of fresh distiller's grains; the moisture content of the fresh distiller's grains is 58-60%;

[0093] The weight ratio of each raw material in the composite strain is: 5 parts of Aspergillus niger (CICC2139AS3.324), 4 parts of Aspergillus oryzae (CICC2329 Huyao 3.042), and 1 part of Bacillus subtilis (BNCC188080);

[0094] The number of living bacteria of each raw material in the composite fermentation agent: Aspergillus niger (CICC2139 AS3.324) 1.10×10 10 ~1.15×10 10 cfu / g, Aspergillus oryzae (CICC2329 Shanghai Brewed 3.0...

Embodiment 3

[0096] Embodiment 3, the preparation method of the protein feed optimized by composite bacteria solid-state aerobic fermentation

[0097] Such as figure 1 , 2 , 3, 4,

[0098] The preparation method of the protein feed optimized by the solid-state aerobic fermentation of the compound bacteria firstly prepares a special feed fermentation bed with a uniform upper and lower temperature, and then uses the compound bacteria to carry out the solid-state aerobic fermentation of the protein feed to obtain the protein optimized by the solid-state aerobic fermentation of the compound bacteria feed;

[0099] [1] The structure of the dedicated feed fermentation bed with uniform upper and lower temperature includes a canopy fermentation tank 1 and an air-water circulation system 2;

[0100] The structure of the canopy fermenter 1: comprises a long trough container 4 with an upper opening, the bottom of the long trough container 4 is an inclined inclined plane tank bottom 5, between the...

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Abstract

The present invention discloses a protein feed optimized by compound micro-organism solid-state aerobic fermentation and a preparation method thereof, and belongs to a compound micro-organism solid-state aerobic fermentation technology not needing material turning. The protein feed comprises the following raw materials: cottonseed meal, rapeseed meal, fresh baijiu distiller's grains, aspergillus niger (CICC2139AS3.324), aspergillus oryzae (CICC2329 Huniang 3.042) and bacillus subtilis (BNCC188080). The preparation method is as follows: firstly, a special feed fermentation bed with upper and lower uniform temperatures is prepared and then the compound micro-organisms are used to conduct twice fermentation two-stage solid-state aerobic fermentation to obtain the protein feed optimized by thecompound micro-organism solid-state aerobic fermentation. Advantages are as follows: the preparation method has very good synergistic effects on the cottonseed meal, rapeseed meal, baijiu distiller'sgrains, aspergillus oryzae and bacillus subtilis, the fermentation method uses a special fermentation device, fermentation micro-organisms can be added conveniently and quickly during the fermentation process, the preparation method enables fermentation effects of the cottonseed meal and rapeseed meal to be better and feeding effects are better.

Description

technical field [0001] The invention belongs to the technical field of feed fermentation, in particular to a composite bacteria solid-state aerobic fermentation technology which can be fermented in two stages and can not be turned over during the fermentation period. Background technique [0002] In recent years, with the large-scale promotion of "corn-soybean meal" rations, the demand for soybean meal is increasing. In 2017, my country's total soybean demand reached 110.79 million tons, but domestic soybean production did not exceed 15 million tons per year. In 2017, my country imported 95.53 million tons of soybeans, an increase of 11.62 million tons compared with 2016. The lack of protein feed resources has gradually become a key factor restricting the development of animal husbandry in my country, and the impact of the international environment and price fluctuations have brought great resistance to the development of feed industry and animal husbandry in China. Therefo...

Claims

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Application Information

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IPC IPC(8): A23K10/12C12M1/00C12M1/04
CPCA23K10/12C12M23/02C12M29/06
Inventor 彭忠利高彦华柏雪陈仕勇曾钰
Owner SOUTHWEST UNIVERSITY FOR NATIONALITIES
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