A strain of Bacillus Velez, its microecological preparation and its preparation method
A technology of Bacillus Velez and microecological preparations, applied in the field of microecological preparations, can solve the problem of fish disease prevention and control without Bacillus Velez, the disease prevention and control effect of freshwater fish that has not been seen, and the prevention and treatment of freshwater fish. Fish pathogenic bacteria have antagonistic effects and other issues
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment Construction
[0043] The present invention will be described in further detail below in conjunction with the embodiments and accompanying drawings.
[0044] The chemical reagents used in the examples of the present invention were obtained through conventional commercial channels unless otherwise specified.
[0045] (1) Collection, isolation, purification and screening process of Bacillus veleisi BvL03
[0046] Collect sediment samples from fish ponds in Qiaokou Town, Wangcheng District, Changsha City, Hunan Province, weigh 5 g of sediment and add it to 45 mL of sterile water, shake it with a vortex shaker, and then dilute it with a 10-fold gradient dilution method. diluted to 10 -4 g / mL, 10 -5 g / mL, 10 -6 g / mL, use a pipette to take 100 μL onto the LB plate, spread it evenly with a disposable spreader, set up 3 parallels, culture at 30 ℃ overnight, pick a single clone for purification, and purify 3 times, Pick a single clone of the purified strain and insert it into LB liquid medium, c...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com