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A strain of Bacillus Velez, its microecological preparation and its preparation method

A technology of Bacillus Velez and microecological preparations, applied in the field of microecological preparations, can solve the problem of fish disease prevention and control without Bacillus Velez, the disease prevention and control effect of freshwater fish that has not been seen, and the prevention and treatment of freshwater fish. Fish pathogenic bacteria have antagonistic effects and other issues

Active Publication Date: 2022-05-20
乾胜(深圳)科创集团有限公司
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0005] Although there have been many research reports on Bacillus velezensis as an important biocontrol bacterium and probiotic, and there are also research reports on the antagonism of pathogenic bacteria in seawater fish, but so far, it has not been seen Involving the research reports that the pathogenic bacteria of freshwater fish have an antagonistic effect; there is no report that it has a preventive effect on the disease of freshwater fish; similarly, there is no report that Bacillus Velez can be used to make bacterial agents or microecological preparations Reports on fish disease control

Method used

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  • A strain of Bacillus Velez, its microecological preparation and its preparation method
  • A strain of Bacillus Velez, its microecological preparation and its preparation method
  • A strain of Bacillus Velez, its microecological preparation and its preparation method

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Embodiment Construction

[0043] The present invention will be described in further detail below in conjunction with the embodiments and accompanying drawings.

[0044] The chemical reagents used in the examples of the present invention were obtained through conventional commercial channels unless otherwise specified.

[0045] (1) Collection, isolation, purification and screening process of Bacillus veleisi BvL03

[0046] Collect sediment samples from fish ponds in Qiaokou Town, Wangcheng District, Changsha City, Hunan Province, weigh 5 g of sediment and add it to 45 mL of sterile water, shake it with a vortex shaker, and then dilute it with a 10-fold gradient dilution method. diluted to 10 -4 g / mL, 10 -5 g / mL, 10 -6 g / mL, use a pipette to take 100 μL onto the LB plate, spread it evenly with a disposable spreader, set up 3 parallels, culture at 30 ℃ overnight, pick a single clone for purification, and purify 3 times, Pick a single clone of the purified strain and insert it into LB liquid medium, c...

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Abstract

A strain of Bacillus Velez, its microecological preparation and its preparation method, the Bacillus Velez is preserved in the China Center for Type Culture Collection, the strain preservation number is CCTCC NO: M 2019218, and the classification name is: Velez Bacillus BvL03, Latin scientific name: Bacillus velezensis BvL03. The microecological preparation suitable for aquaculture made by this strain has a broad-spectrum inhibitory effect on fish pathogenic bacteria through feed addition, water splashing or intraperitoneal injection, and the protection rate reaches 85%. After acting on fish, it can effectively It has the best protective effect against the infection of fish pathogens such as Aeromonas hydrophila on grass carp, crucian carp and other freshwater fish, especially grass carp.

Description

technical field [0001] The invention relates to the field of microecological preparations, in particular to a strain of Bacillus velez, its probiotics and a preparation method thereof. Background technique [0002] In recent years, in the process of aquaculture, bacterial diseases have occurred frequently and spread widely. Among them, explosive hemorrhagic disease is the most harmful to fish. Once this disease breaks out, it will cause very serious economic losses. However, the prevention and treatment of bacterial diseases mainly relies on antibiotics and chemical drugs, but the methods and dosages used are unscientific, and there are also many hidden dangers, such as the emergence of mutant drug-resistant strains, the destruction of the ecological environment of water bodies, and disturbance of the intestinal tract of aquaculture animals. The ecological balance of microorganisms can cause hidden dangers in the safety of aquatic products and reduce the immunity of aquacul...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/742A61P31/04A23K10/18A23K50/80C12R1/07
CPCC12N1/20A61P31/04A23K10/18A23K50/80A61K2035/115C12R2001/07C12N1/205Y02A40/818
Inventor 夏立秋曹丽娜易敢峰丁学知
Owner 乾胜(深圳)科创集团有限公司
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