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The medical application of compound pyrogallol in anti-Neospora canis

A technology of Neospora caninum and a compound, applied in the field of antiparasitic drugs, can solve the problems of lack of specific drugs and the like, and achieve the effect of significant curative effect

Inactive Publication Date: 2021-12-03
JILIN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] So far, although co-trimoxazole, tetracycline, pyrimethamine and other drugs have certain effects, there is currently a lack of specific drugs, so there is an urgent need to develop new drugs

Method used

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  • The medical application of compound pyrogallol in anti-Neospora canis
  • The medical application of compound pyrogallol in anti-Neospora canis
  • The medical application of compound pyrogallol in anti-Neospora canis

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Experimental program
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Effect test

Embodiment 1

[0022] The present invention takes the ROS inducer Pyrogallol as an example to prove its therapeutic effect on neospora invasion of cells, intracellular proliferation and infection of mouse heart, liver, spleen, lung, kidney and brain.

[0023] The pathway of Pyrogallol acting on primary mouse peritoneal macrophages: After using Pyrogallol to activate the NLRP3 pathway of host cells, observe its effect on the host cells infected by Neospora, and conduct biostatistical analysis on the data obtained from the experiment. After the cells were infected with Neospora tachyzoites (tachyzoites: cells = 1:1) for 6 hours, Pyrogallol (30 µM) was added to the medium and incubated with the host cells for 24 hours and 48 hours. The results showed that: at 24 hours, intracellular The percentage of parasites decreased by 83.84% (P figure 1 ).

Embodiment 2

[0025] Use of anti-Neospora drugs

[0026] 1. The blocking effect of ROS inducer on the invasion of Neospora cells and the inhibition effect of intracellular parasite proliferation

[0027] The present invention takes the ROS inducer Pyrogallol as an example to observe its blocking effect on neospora invasion of primary peritoneal macrophages of mice and its inhibitory effect on the proliferation of intracellular parasites, and perform biostatistical analysis on the data obtained from the test . The results showed that Pyrogallol had no concentration-dependent effect on inhibiting the invasion of Neospora cells. After Neospora tachyzoites were infected for 6 hours, they were treated with 15µM, 30µM, and 60µM Pyrogallol for 24 hours respectively. The number of tachyzoites decreased by 36.5%, 37.7% and 32% respectively (P figure 2 ).

[0028] , Inhibition effect of ROS inducer on intracellular proliferation of Neospora

[0029] The present invention takes the ROS inducer Pyro...

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Abstract

The invention discloses the medical application of the compound pyrogallol in anti-Neospora canis, and can be made into a novel anti-Neospora caninum drug, and its anti-Neospora effect on neospores is confirmed by two aspects of animal experiments acting on host cells and mouse models Therapeutic effect of worm, can be made into the chemical medicine that suppresses Neospora cell infection and mouse model heart, liver, spleen, lung, kidney, brain tissue infection, has remarkable curative effect; The present invention provides for the treatment of Neospora The new candidate drug target-reactive oxygen species (ROS) and the new drug candidate-pyrogallol lay the foundation for the search for new drugs against neosporosis in the future.

Description

technical field [0001] The invention provides the medical application of the compound pyrrogallol in anti-Neospora canis, which can be prepared into a novel anti-Neospora caninum drug and belongs to the technical field of anti-parasitic drugs. Background technique [0002] Neosporiasis is caused by Neospora canis ( Neospora caninum ) is a fatal protozoan disease caused by infection. Its host range is wide, including most mammals. Dogs and canids are its final hosts. Intermediate hosts include cattle, sheep, deer, horses, dogs, rabbits and other animals. It mainly causes abortion of pregnant animals, newborn fetus movement and Nervous system diseases, among them the most serious harm to cattle, have caused serious economic losses to the cattle industry in the world. Based on the harm caused by neosporosis to animal husbandry and aquaculture, the research on this disease has gradually been paid attention to by the veterinary field in recent years. [0003] So far, although ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/05A61P33/02
Inventor 张西臣李璐李建华宫鹏涛王晓岑张楠马赫然李新李赫杨举
Owner JILIN UNIV
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