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Application and kit of hsa-mir-15b-5p as a molecular marker

A technology of hsa-mir-15b-5p and molecular markers, applied in the field of molecular biotechnology and its applications, to achieve the effects of high reliability, relief of altitude sickness, and convenient sample acquisition

Active Publication Date: 2022-04-01
ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few reports on the correlation between circulating microRNAs and AMS susceptibility.

Method used

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  • Application and kit of hsa-mir-15b-5p as a molecular marker
  • Application and kit of hsa-mir-15b-5p as a molecular marker
  • Application and kit of hsa-mir-15b-5p as a molecular marker

Examples

Experimental program
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Effect test

Embodiment 1

[0041] Example 1 Correlation between the expression of microRNA in saliva samples and the risk of AMS

[0042] 1. Material and Specimen Collection Description

[0043] The saliva samples of AMS-susceptible persons were collected from AMS patients who entered the plateau rapidly before entering the plateau, a total of 75 cases. The saliva samples of normal people were collected from 49 cases of normal healthy people who entered the plateau rapidly before entering the plateau. The diagnosis of AMS was confirmed by the International Common Diagnostic Criteria-Lake Lewis Score. All the groups did not take any preventive medicine before taking saliva. A total of 10ml of saliva was collected from each sample in a sterile, enzyme-free 50ml centrifuge tube. The clinical case characteristics of the patients are shown in Table 1. This study has been approved by the Medical Ethics Committee of the Army Military Medical University (Third Military Medical University), and all specimens...

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Abstract

The present invention relates to the application of hsa-miR-15b-5p as a microRNA marker in the saliva of people susceptible to acute altitude sickness screening, and the microRNA is used in the preparation of reagents or kits for screening people susceptible to acute altitude sickness, saliva The expression of hsa-miR-15b-5p in the specimen was down-regulated compared with the expression in the control. It also provides a kit for detecting the hsa-miR-15b-5p molecule in the plain saliva of the tested human by the real-time fluorescent quantitative PCR method, and predicts the risk of acute altitude sickness by its expression level. As a molecular marker, the microRNA has the characteristics of strong specificity and high sensitivity, non-invasive and convenient acquisition of tested samples, and is suitable for large-scale screening of people susceptible to acute altitude sickness in the plains.

Description

technical field [0001] The invention belongs to the field of molecular biotechnology and its application. The invention relates to an application and a kit for hsa-miR-15b-5p as a microRNA molecular marker for screening the saliva of persons susceptible to acute altitude sickness. Screen the risk of acute altitude sickness by screening the expression level of hsa-miR-15b-5p in saliva, so as to evaluate the human body's susceptibility to acute altitude sickness and reduce the risk of acute altitude sickness in humans. Background technique [0002] Acute mountain sickness (AMS) occurs in plain people who have lived in low-altitude areas for a long time, 1-3 days after entering the plateau quickly without adapting to the new environment, including headache, dizziness, fatigue, insomnia , Emotional restlessness, flatulence, diarrhea and vomiting and a series of symptoms, among which severe headache is a typical symptom of AMS. In geography, a vast area with an altitude higher t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883
Inventor 黄河张二龙高钰琪徐刚何姝董华平柯贤峰陈建李鹏孙滨达
Owner ARMY MEDICAL UNIV
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