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A phenol-degrading bacterium producing biosurfactant and its application

A technology for phenol-degrading bacteria and surfactants, which is applied in the field of phenol-degrading bacteria, can solve the problems of increasing the production cost of biosurfactants, the strains cannot adapt to the solution environment of phenols, and the conditions of biosurfactants are harsh, and achieve good degradation. effect, strong emulsification, solubilization-promoting effect

Active Publication Date: 2022-04-26
WUHAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, the patented technology of "a method for producing biosurfactant by a strain of Pseudomonas" (CN102732573A), the advantage of this technology is that the rate of biosurfactant production is relatively fast, but the strain itself cannot adapt to the solution environment containing phenols; " A strain of Pseudomonas aeruginosa that produces biosurfactants" (CN101705200A) patented technology, the advantage of this technology is high yield, but the conditions for producing biosurfactants are relatively harsh, which increases the production cost of biosurfactants

Method used

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  • A phenol-degrading bacterium producing biosurfactant and its application
  • A phenol-degrading bacterium producing biosurfactant and its application
  • A phenol-degrading bacterium producing biosurfactant and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Acquisition and identification of strain (Pseudomonas plecoglossicida) BPH-3. The steps of acquisition and identification of bacterial strain (Pseudomonasplecoglossicida) BPH-3 are:

[0019] Step 1. Sample collection and enrichment culture

[0020] The bacteria source was obtained from the activated sludge in the biochemical tank of Wuhan Petrochemical Wastewater Treatment Plant.

[0021] Get 10ml of the activated sludge and place it in an inorganic salt medium with a paraffin concentration of 1vol%, shake it at a constant temperature in a shaker at 150r / min and 30°C, cultivate it for 48h, and then transfer it to a new medium with a paraffin concentration of 1vol%. In the inorganic salt medium, a culture solution was obtained.

[0022] The formula of described inorganic salt culture medium is: (NH 4 ) 2 SO 4 1g, KH 2 PO 4 1g, K 2 HPO 4 1g, NaCl, MgSO 4 2H 2 0 is 0.2g, FeSO 4 ·7H 2 O is 0.02g, anhydrous CaCl 2 0.02g, H 2 O is 1L. The pH value of the mediu...

Embodiment 2

[0030] Effect of initial concentration of phenol on the degradation performance of strain (Pseudomonas plecoglossicida) BPH-3. The bacterial strain (Pseudomonas plecoglossicida) BPH-3 was inoculated into the inorganic salt medium with the initial concentration of phenol being 200, 400, 600, 800, 1000, 1100 mg / L respectively; After culturing in the bed for 12 hours, the effect of the initial concentration of phenol on the degradation performance of strain (Pseudomonas plecoglossicida) BPH-3 was determined. The results are shown in Table 1.

[0031]Table 1 Effect of the initial concentration of phenol on the degradation performance of strain (Pseudomonas plecoglossicida) BPH-3

[0032]

[0033]

[0034] It can be seen from Table 1 that bacterial strain (Pseudomonas plecoglossicida) BPH-3 has a 100% degradation rate to an initial concentration of 200-600 mg / L within 12 hours, and a 100% degradation rate to a phenol initial concentration of 800 mg / L within 18 hours .

[0...

Embodiment 3

[0037] Effect of pH on the degradation of phenol by strain (Pseudomonas plecoglossicida) BPH-3. The bacterial strain (Pseudomonas plecoglossicida) BPH-3 was inoculated into the inorganic salt medium whose initial concentration of phenol was 600 mg / L, and the pH value of the inorganic salt medium was adjusted to 3, 4, 5, 6, 7, 8, 9, 10 and 11, cultivated in a constant temperature shaker at a temperature of 30° C. and a rotation speed of 130 r / min for 12 hours, and measured the influence of pH value on the degradation of phenol by strain (Pseudomonas plecoglossicida) BPH-3. The results are shown in Table 2.

[0038] Table 2 Effect of pH value on degradation of phenol by strain (Pseudomonas plecoglossicida) BPH-3

[0039] pH value Degradation rate 3 1.35 4 7.49 5 57.94 6 99.45 7 100 8 99.15 9 69.99 10 45.79 11 2.27

[0040] It can be seen from Table 2 that when the pH value is 7, the degradation rate of phenol by stra...

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Abstract

The present invention relates to a phenol-degrading bacterium producing biological surfactant and its application. Its technical scheme is: the provided bacterial strain is Pseudomonas plecoglossicida BPH-3, which is preserved by the China Center for Type Culture Collection, and the preservation number is: CCTCC NO: M 2019144, and the preservation date is March 14, 2019 , the GenBank accession number of the 16S rDNA of the Pseudomonas plecoglossicida (Pseudomonas plecoglossicida) BPH-3 is MK575471, and the Pseudomonas (Pseudomonas plecoglossicida) BPH-3 is a gram-negative bacteria, and the colony is milky white, circular , smooth surface, neat edges; negative under the microscope after Gram staining, the strain was short rod-shaped. The phenol-degrading bacteria produced a glycolipid biosurfactant in the process of fermentation and degradation, which can promote the solubilization, emulsification, wetting and penetration of long-chain alkanes, and can effectively degrade phenol in coking wastewater and petrochemical wastewater.

Description

technical field [0001] The invention belongs to the technical field of phenol-degrading bacteria. In particular, it relates to a phenol-degrading bacterium producing biosurfactant and its application. Background technique [0002] With the rapid development of petrochemical, coking, plastics and other industries, various phenolic wastewaters have also increased accordingly. Due to the high toxicity of phenols, and it involves the growth and reproduction of aquatic organisms and pollutes water resources, it is necessary to treat phenolic wastewaters. Treatment Take effective measures, and the treatment of phenolic industrial wastewater has become one of the problems to be solved urgently. [0003] Using physical and chemical methods to treat phenolic wastewater may not only cause secondary pollution, but also be uneconomical due to problems such as solvent loss or high energy consumption. The biochemical treatment process is generally simple to operate and low in operating c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P1/04C02F3/34C12R1/38C02F101/34
Inventor 李文兵黄慧王光华张政陈铁邓萌
Owner WUHAN UNIV OF SCI & TECH
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