The Culture Method of Callus Induction and Regeneration of Mature Embryo of Goose View Grass

Abstract

The invention discloses a method for inducing and regenerating callus of mature embryos of A. argentina, which comprises the following steps: (1) disinfection and sterilization of explants, (2) culturing of induced callus, (3) ) differentiation culture of callus, (4) rooting culture of induced differentiated shoots, (5) transplanting culture. The advantage of the present invention is that each seed harvested when Goose View Grass is mature contains a mature embryo, and the seeds can be stored for many years. As long as there are seeds, callus can be induced at any time, and the time limit for taking materials is lifted; mature embryos can induce callus During the process, it is not necessary to peel off the mature embryos, and the sterilized seeds can be used directly, which simplifies the operation steps and reduces the difficulty of operation; by adding a certain proportion of 2,4‑D and KT, the callus can be The induction rate of tissue is increased to 78%; in the process of differentiation culture to regeneration culture, by adding a certain concentration ratio of 6‑BA and KT, the callus differentiation rate can reach more than 90%, and the rooting rate of differentiated seedlings can reach more than 97%. .

Description

Technical field: [0001] The invention relates to the field of biotechnology, in particular to a culture method for inducing and regenerating mature embryo callus of A. Background technique: [0002] Goose view grass is a species under the genus Goose view grass of Gramineae. It is a common herb that can be used as feed for livestock. It has a large grass yield and high edibility, and it has the high yield, Good genes such as resistance to diseases and insect pests and stress resistance can be used as valuable resources for breeding new varieties of wheat crops. [0003] As an excellent grass species with important application value, the construction of an efficient regeneration system is crucial to the establishment of a molecular genetic system, and it is also the technical basis for the study of gene function and transgenic research using gene editing technology. At present, there is only one case of research reports on the regeneration of Goosegrass, a high-frequency cul...

AI Technical Summary

Problems solved by technology

However, there are the following problems in the cultivation of callus induction and regeneration using young embryos as explants: 1. The time for taking materials is limited by the growth period, and the young embryos of Goose View Grass can only be obtained from live embryos in the late booting stage and early heading stage of Goose View Grass. It is impossible to obtain explants at any time throughout the year, and the suitable time for taking materials can only last for about 20 days, which is extremely unfavorable for the research on the construction of an efficient regeneration system of Goose View Grass; 2. The young embryos of Goose View Grass are very small, about 0.5mm×0.5mm, it needs to be peeled off from immature seeds under sterile conditions with the help of a dissecting microscope, and the immature seeds cannot be damaged, so the operator is required to have a high level of technology, the operation is difficult, and it is not conducive to large Scale expansion

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