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Liquid fermentation culture medium and fermentation culture method for gloeostereum incamatum S.Ito et Imai

A technology of liquid fermentation and solid culture medium, applied in the field of liquid fermentation medium of Ulmus ulm, can solve problems such as time-consuming and laborious cultivation of Ulmus, and achieve the effects of shortening fermentation time, long growth period and increasing polysaccharide content.

Active Publication Date: 2019-05-31
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Traditional ulmus cultivation is time-consuming and labor-intensive. Compared with traditional cultivation techniques, the application of liquid submerged fermentation technology can quickly, stably, and large-scale obtain medicinal fungal mycelia and active secondary metabolites. Today, liquid submerged fermentation production has become An Important Means of Exploitation of Medicinal Fungi Resources

Method used

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  • Liquid fermentation culture medium and fermentation culture method for gloeostereum incamatum S.Ito et Imai
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  • Liquid fermentation culture medium and fermentation culture method for gloeostereum incamatum S.Ito et Imai

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Embodiment 1

[0039]A preparation method of a traditional Chinese medicine enzyme extract is as follows: put the traditional Chinese medicine mulberry leaves, mugwort leaves and purslane into an enzymolysis tank according to the following parts by weight: 30 parts of mulberry leaves, 40 parts of mugwort leaves and 30 parts of purslane, and add distilled water to obtain The medicine mixture, the weight ratio of traditional Chinese medicine and distilled water is 1:10, then add cellulose degrading enzyme and protein degrading enzyme, the adding amount of cellulose degrading enzyme is 1% of the total weight of the medicine mixture, and the adding amount of protein degrading enzyme is the total weight of the medicine mixture 0.5%, and then degraded at a constant temperature of 50°C for 24 hours, and finally the filtrate was taken out and filtered, which was the traditional Chinese medicine enzyme extract.

Embodiment 2

[0041] A preparation method of a traditional Chinese medicine enzyme extract is as follows: put the traditional Chinese medicine mulberry leaves, mugwort leaves and purslane into an enzymolysis tank according to the following parts by weight: 30 parts of mulberry leaves, 40 parts of mugwort leaves and 30 parts of purslane, and add distilled water to obtain The medicine mixture, the weight ratio of traditional Chinese medicine and distilled water is 1:10, then add cellulose degrading enzyme and protein degrading enzyme, the adding amount of cellulose degrading enzyme is 1.5% of the total weight of the medicine mixture, and the adding amount of protein degrading enzyme is the total weight of the medicine mixture 1%, and then degrade at a constant temperature of 50°C for 24 hours, and finally take out and filter the filtrate, which is the traditional Chinese medicine enzyme extract.

Embodiment 3

[0043] A preparation method of a traditional Chinese medicine enzyme extract is as follows: put the traditional Chinese medicine mulberry leaves, mugwort leaves and purslane into an enzymolysis tank according to the following parts by weight: 30 parts of mulberry leaves, 40 parts of mugwort leaves and 30 parts of purslane, and add distilled water to obtain The medicine mixture, the weight ratio of traditional Chinese medicine and distilled water is 1:10, then add cellulose degrading enzyme and protein degrading enzyme, the adding amount of cellulose degrading enzyme is 1.2% of the total weight of the medicine mixture, and the adding amount of protein degrading enzyme is the total weight of the medicine mixture 0.8% of 0.8%, and then degrade at a constant temperature of 50°C for 24 hours, and finally take out and filter the filtrate, which is the traditional Chinese medicine enzyme extract.

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Abstract

The invention discloses a liquid fermentation culture medium and a fermentation culture method for gloeostereum incamatum S.Ito et Imai. According to the method, the proper culture medium and cultureconditions are selected, and traditional Chinese medicine mulberry leaves, folium artemisiae argyi and portulaca oleracea extracts promote the growth of mycelia of gloeostereum incamatum S.Ito et Imai, so that the liquid fermentation time of the gloeostereum incamatum S.Ito et Imai is shortened; meanwhile, the biomass of the mycelia of the gloeostereum incamatum S.Ito et Imai and the content of polysaccharide substances are increased.

Description

technical field [0001] The invention relates to the technical field of fermentation culture, in particular to a liquid fermentation culture medium of ulmus fungus and a method for liquid fermentation and cultivation of ulmus fungus. Background technique [0002] Elm ( Gloeostereum incamatum S.Ito et Imai) is scientifically known as the red gum bacillus, aliased as ulm mushroom, meat mushroom, gum bacillus, succulent sticky bacteria, meat red gum bacillus, Ulmus spp., sticky bacillus, Radix bacteria. Belonging to the subphylum Basidiomycotina, Hymenomycetes, Aphyllophorales, Corticiaceae, and the Gloeostereum ) Fungus with both medicinal and edible uses. It mostly grows on the trunks, stumps, dead branches or tree holes of elm (Ulmus pumila) and spring elm (U. propinqua), and can also grow on sugar maple trees. It is mainly distributed in Liaoning, Heilongjiang, Jilin and other places, as well as Hokkaido in Japan. [0003] The fruit body of ulmus elm is rich in protei...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
Inventor 刘宇王建瑞谷丽凡彭炜航程显好崔永进田伟
Owner LUDONG UNIVERSITY
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