Sandwich type electrochemical sensor constructed based on boric acid affinity, preparation method and application thereof

A sandwich and electrochemical technology, applied in the field of sensor detection, can solve the problems of poor selectivity, few detection methods, and low NADH detection sensitivity, and achieve the effects of low cost, high sensitivity and strong anti-interference ability.

Active Publication Date: 2019-05-17
ANHUI NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a sandwich-type electrochemical sensor based on boric acid affinity construction and its preparation method and application, which solves the problems of low detection sensitivity, few detection methods and poor selectivity of traditional NADH

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  • Sandwich type electrochemical sensor constructed based on boric acid affinity, preparation method and application thereof
  • Sandwich type electrochemical sensor constructed based on boric acid affinity, preparation method and application thereof
  • Sandwich type electrochemical sensor constructed based on boric acid affinity, preparation method and application thereof

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preparation example Construction

[0027] The invention provides a method for preparing a sandwich-type electrochemical sensor based on boronic acid affinity, the preparation method comprising:

[0028] (1) Preparation of RGO / GCE: Add GO solution dropwise on the GCE electrode, and then conduct electrodeposition in PBS solution to obtain the RGO / GCE electrode;

[0029] (2) Preparation of PAPBA / RGO / GCE: Mix APBA solution, HCl solution and NaF solution to obtain mixed solution A, and perform cyclic voltammetry scanning on the above-mentioned RGO / GCE electrode in mixed solution A to obtain PAPBA / RGO / GCE electrode;

[0030] (3) Add BSA solution dropwise on the PAPBA / RGO / GCE electrode to obtain a BSA / PAPBA / RGO / GCE electrode;

[0031] (4) PBS solution, NADH and FcBA solutions were mixed to obtain mixed solution B, and BSA / PAPBA / RGO / GCE was placed in mixed solution B for reaction to obtain a sandwich type electrochemical sensor.

[0032] In a preferred embodiment of the present invention, in order to further increas...

Embodiment 1

[0048] (1) Preparation of RGO / GCE: Add 8 μL GO solution (concentration: 0.8 mg / mL) dropwise on the GCE electrode, and then conduct electrodeposition in PBS solution (pH: 6.8) (potential range: -1.4VV, time: 715s), the RGO / GCE electrode is obtained;

[0049] (2) Preparation of PAPBA / RGO / GCE: Mix APBA solution, HCl solution and NaF solution to obtain mixed solution A (concentration of APBA solution is 9mmol / L, concentration of HCl solution is 0.9mol / L, concentration of NaF solution 4mmol / L), the above-mentioned RGO / GCE electrode was subjected to cyclic voltammetry scanning in the mixed solution A (the potential range was -0.3V~0.5V, and the scanning speed was 0.09V / s), and the PAPBA / RGO / GCE electrode was obtained ;

[0050] (3) Add 8 μL of BSA solution (volume concentration: 4%) dropwise on the PAPBA / RGO / GCE electrode to obtain a BSA / PAPBA / RGO / GCE electrode;

[0051] (4) Mix PBS solution, NADH and FcBA solution to obtain mixed solution B (wherein, the concentration of FcBA sol...

Embodiment 2

[0053] (1) Preparation of RGO / GCE: Add 10 μL GO solution (concentration: 1 mg / mL) dropwise on the GCE electrode, and then conduct electrodeposition in PBS solution (pH: 7) (potential range: -1.4V, time: 720s ), namely the RGO / GCE electrode;

[0054] (2) Preparation of PAPBA / RGO / GCE: Mix APBA solution, HCl solution and NaF solution to obtain mixed solution A (wherein, the concentration of APBA solution is 10mmol / L, the concentration of HCl solution is 1mol / L, and the concentration of NaF solution Concentration is 5mmol / L), and the above RGO / GCE electrode is subjected to cyclic voltammetry scanning in the mixed solution A (the potential range is -0.4V~0.6V, and the scanning speed is 0.1V / s), that is, PAPBA / RGO / GCE electrode;

[0055] (3) Add 10 μL of BSA solution (volume concentration: 5%) dropwise on the PAPBA / RGO / GCE electrode to obtain a BSA / PAPBA / RGO / GCE electrode;

[0056] (4) Mix PBS solution, NADH and FcBA solution to obtain mixed solution B (concentration of FcBA solut...

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Abstract

The invention discloses a sandwich type electrochemical sensor constructed based on boric acid affinity, a preparation method and application thereof. The preparation method comprises the steps of: dropping GO solution on a GCE electrode, and then, performing electric deposition in PBS solution, so that an RGO / GCE electrode is obtained; mixing APBA solution, HCl solution with NaF solution, so thatmixed solution A is obtained, and performing cyclic voltammetry scanning of the RGO / GCE electrode in the mixed solution A, so that a PAPBA / RGO / GCE electrode is obtained; dropping BSA solution on thePAPBA / RGO / GCE electrode, so that a BSA / PAPBA / RGO / GCE electrode is obtained; and, mixing the PBS solution, NADH with FcBA solution, so that mixed solution B is obtained, and reacting by putting the BSA / PAPBA / RGO / GCE in the mixed solution B, so that the sandwich type electrochemical sensor is obtained. The problem that the traditional NADH detection is low in sensitivity, few in detection method, poor in selectivity and the like can be solved.

Description

technical field [0001] The invention relates to the field of sensor detection, in particular to a sandwich-type electrochemical sensor constructed based on boric acid affinity and its preparation method and application. Background technique [0002] Nicotinamide adenine dinucleotide (NADH), an important coenzyme involved in metabolic processes, widely exists in the cells of organisms. It can stimulate the production of neurotransmitters such as dopamine, norepinephrine, and serotonin in cells, and at the same time provide energy for the immune system and have a repairing effect on the DNA system. Therefore, the development of sensors for NADH detection has become a research topic in recent years. Because the oxidation of NADH on the electrode surface requires a high overpotential, its oxidation on conventional solid electrodes is highly irreversible, and NADH is easy to accumulate on the electrode surface, hindering electron transfer and limiting the sensitivity of the analy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/48
Inventor 阚显文刘芬
Owner ANHUI NORMAL UNIV
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