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A method for improving the protective effect of creatine on excitotoxicity

A technology of neurotoxicity and excitability, applied in the field of biomedicine, can solve the problems of not mentioning Cr, limited therapeutic effect, ignoring the catalytic function of CK, etc., and achieve the effect of improving the protective effect

Active Publication Date: 2021-07-02
王铁鹏
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  • Abstract
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  • Application Information

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Problems solved by technology

What is the reason for the limited therapeutic effect of Cr? There is no targeted research report
Literature survey found that in many researches trying to improve neurodegeneration through Cr, the researchers mainly focused on factors such as dose, time, and administration method of Cr, but did not mention whether the addition of Cr can really be catalyzed to form PCr, ignoring whether the catalytic function of CK is normal

Method used

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  • A method for improving the protective effect of creatine on excitotoxicity
  • A method for improving the protective effect of creatine on excitotoxicity
  • A method for improving the protective effect of creatine on excitotoxicity

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Embodiment Construction

[0023] The technical solution of the present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.

[0024] The technical terms used in the present invention are put into Chinese and English contrast: creatine (Creatine, Cr), creatine kinase (Creatine kinase, CK), nitrosoglutathione (S-Nitrosoglutathione, GSNO), GSNO reductase ( S-nitrosoglutathione reductase, GSNOR), nerve cell cytoplasmic creatine kinase (Creatine kinasebrain type, CKBB), nerve cell mitochondrial creatine kinase (Mitochondrial creatine kinase, CKMT), phosphocreatine (phosphocreatine, PCr), 2, 4-Dinitrofluorobenzene (2,4-Dinitrofluorobenzene, DNFB), Cerebellar granule neuron (CGN), Alzheimer's disease (Alzheimer's disease, AD), Parkinson's disease (Parkinson's disease , PD), Huntington's disease (Huntington's disease, HD), amyotrophic lateral sclerosis (Amyotrophic lateral sclerosis, ALS).

[0025] 1. Experimental method

[0026] 1.1 Exog...

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Abstract

The invention discloses a method for improving the protective effect of creatine on excitatory neurotoxicity, establishes an excitotoxic model of SH‑SY5Y cells treated with exogenous nitric oxide donor GSNO, and uses the model to prove that simple muscle Acid factor has a protective effect on neuroexcitotoxicity, but the effect is limited. In the SH‑SY5Y cell model treated with GSNO, the two neural creatine kinases CKBB and CKMT were nitrosylated, and the protein content and enzyme activity of the two were significantly reduced after modification; overexpression denitrosylation The modification enzyme GSNOR can significantly reduce the nitrosylation modification of CKBB and CKMT, and can significantly restore the content and activity of CKBB and CKMT. The present invention can significantly enhance the protective effect of creatine on excitotoxicity in combination with the denitrosylation modification scheme of neural creatine kinase.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and relates to a method for improving the protective effect of creatine on excitatory neurotoxicity, in particular to a denitrosylation modification method of neural creatine kinase (CKBB and CKMT) in enhancing creatine Drug application in neuroprotective effect. Background technique [0002] Neurodegenerative diseases are a group of neurological diseases that result in progressive functional variation and death of nerve cells, leading to abnormal behavior and dysfunction of patients, and premature death, including Alzheimer's disease (AD), Parkinson's disease disease (PD), Huntington's disease (HD), amyotrophic lateral sclerosis (ALS), etc. Excitotoxicity is a common pathway leading to late neuronal death in the pathogenesis of neurodegenerative diseases. Among them, energy depletion caused by processes such as mitochondrial respiratory chain inhibition, glycolysis inhibition, and energy c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/198A61P25/28
Inventor 王铁鹏陈畅张婷赵博田云云李巧彦张倩孙悦
Owner 王铁鹏
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