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Urine preservation solution, urine collection container, method and kit

A collection container and preservation solution technology, which is applied in the field of cell culture, can solve the problems such as bacteria and fungi contamination, and achieve the effects of prolonging storage time, preventing bacteria and fungi contamination, and simplifying operations

Active Publication Date: 2019-05-17
FUWAI HOSPITAL CHINESE ACAD OF MEDICAL SCI & PEKING UNION MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems in the prior art that cells must be separated immediately after urine collection and are prone to bacterial and fungal contamination, the purpose of the present invention is to provide a urine preservation method that can obtain epithelial cells from urine in a practical manner. liquid, as well as a urine collection container and a method and kit for collecting and preserving urine, so as to preserve the epithelial cells in the urine for a long time and provide sufficient operation time for actually obtaining the epithelial cells

Method used

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  • Urine preservation solution, urine collection container, method and kit
  • Urine preservation solution, urine collection container, method and kit
  • Urine preservation solution, urine collection container, method and kit

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Embodiment approach

[0049] According to a preferred embodiment of the present invention, the rotating device may include:

[0050] Rotate the handle, which is arranged at the bottom of the urine collection container;

[0051] A transmission rotary rod, which is integrally formed of plastic together with the rotary handle, and is provided with a sealing thread at one end connected to the rotary handle; and

[0052] A rubber stopper, which has a circular sheet structure and is arranged on the end of the transmission rotary rod opposite to the rotary handle;

[0053] Wherein the bottle body is provided with a round hole on the bottom surface, the sealing thread and the round hole on the bottom surface are threadedly engaged to seal the bottom surface, and the diameter of the hole in the middle partition is larger than that of the transmission rotary rod diameter and smaller than the diameter of the rubber stopper, the transmission rotary rod passes through the storage liquid tank so that the rubber...

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Abstract

The invention provides a urine preservation solution for obtaining epithelial cells from urine. The urine preservation solution comprises an acid-base balance buffer system, a plurality of ionic components, a plurality of amino acid components and an inhibitor of bacteria or fungi, and does not contain animal-derived components. The invention also provides a urine collection container. The urine collection container comprises a bottle body, a bottle cap and a rotating device, wherein the bottle body comprises a middle partition plate for dividing the bottle body into an upper sample bin and alower preservation solution bin, and the preservation solution bin is pre-filled with the urine preservation solution; the bottle cap is arranged at the top of the urine collection container; the rotating device is arranged to close or open a hole in the middle partition board to close or open communication between the sample bin and the preservation solution bin. The invention further provides amethod for collecting and storing urine for culturing epithelial cells and a corresponding kit. Accordingly, collection of urine samples is simplified, preservation time of the epithelial cells in urine is prolonged, and the epithelial cells are conveniently obtained from the urine.

Description

technical field [0001] The invention relates to the field of cell culture technology, in particular to a urine preservation solution, a urine collection container, a method and a kit for collecting and preserving urine that can be actually used to obtain epithelial cells from urine. Background technique [0002] In recent years, pluripotent induced stem cell technology is an important breakthrough in the field of stem cell research. This technology uses autologous cells to prepare through reprogramming technology, which not only avoids the ethical issues of stem cell application, but also solves the problem of immune rejection of cell therapy. future disease treatments. Collecting and extracting patient sample cells as reprogrammed cells is the initial work for the preparation of pluripotent induced stem cells. At present, skin fibroblasts isolated from patient skin tissue and mononuclear cells isolated from peripheral blood are mostly used. However, there are many problems...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02C12N5/071G01N1/38G01N1/10
Inventor 刘尚雨郑黎晖姚焰
Owner FUWAI HOSPITAL CHINESE ACAD OF MEDICAL SCI & PEKING UNION MEDICAL COLLEGE
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