A kind of preparation method of sialic acid recognition conductive polymer precursor

Abstract

The invention discloses a preparation method of a conductive polymer material capable of specifically identifying tumor cells. The preparation method is characterized by comprising the following steps: (1) in a nitrogen atmosphere, adding 28 ml of toluene, 1.36 g and 10.4 mmol of 3,4-dimethoxy thiophene, 2.60 g and 23.1 mmol of 3-chloro-1,2-propylene glycol and 0.16 g and 0.92 mmol of p-toluene sulfonic acid monohydrate into a double-neck flask, carrying out mixing, and carrying out a reaction for 24 hours at a temperature of 90 DEG C; and (2) evaporating the mixture in the step, and then carrying out column chromatography purification to obtain EDOT-Cl. The preparation method disclosed by the invention has the following advantages: the flexible conductive polymer material capable of specifically identifying tumor cells is synthesized, non-labeling of the tumor cells is realized, non-invasive specific recognition is achieved, and an effective means is provided for screening of malignant tumors, early diagnosis of cancers and instant diagnosis.

Description

technical field [0001] The invention relates to the technical field of polymer materials, in particular to a preparation method for a sialic acid-recognized conductive polymer precursor. Background technique [0002] Sialic acid (SA) is a molecule distributed in the sugar chains on the surface of the cell membrane and concentrated at the end of the sugar chain. In view of its outermost layer (chain end) arrangement and unique anion polarity, SA plays an important role in the physiological and pathological processes of the body. plays an important role. With some exceptions, overexpression of SA on the cell surface is usually a sign of cancer progression. Therefore, the determination of SA is closely related to the diagnosis of cancerous state. Now, there are a variety of SA quantitative kits in circulation on the market, and these multi-stage enzymatic reactions are also accompanied by labeling operations such as fluorescence, which is not only expensive, but usually takes...

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Problems solved by technology

Now, there are a variety of SA quantitative kits in circulation on the market, and these multi-stage enzymatic reactions are also accompanied by labeling operations such as fluorescence, which is not only expensive, but usually takes about a day to determine

In addition, these methods are completely destructive to cells and are not suitable for immediate detection of cells, since these methods require pretreatment with SA enzyme or strong acid treatment to release SA in sugar chains.

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