Evaluation method of rat models suffering from endometriosis, kidney deficiency and blood stasis syndromes

Abstract

The invention discloses an evaluation method of rat models suffering from endometriosis, kidney deficiency and blood stasis syndromes. The evaluation method comprises the following steps: normally feeding experimental rats for one week, then mating with 3-month-old SD male rats according to a ratio of 2:1, and drenching with mifepristone once on the 12 day of pregnancy to cause abortion of pregnant rats, wherein hydroxyurea and the mifepristone are ground into powder and are dissolved in distilled water for drenching; from the next day after stopping of vaginal bleeding of the aborted rats, hitting with a wooden stick to force the rats to swim constantly until a fatigued state, fishing out the experimental rats after swimming, and exposing the experimental rats in air for naturally airing; anesthetizing the rats under an aseptic condition, laparotomizing for shearing intimal tissue blocks, reversely aligning epithelial layers to body walls, closing abdomens, randomly taking 3 ratsafter 4 weeks, laparotomizing again for cutting transplants for inspection, and verifying that the rats have survived. According to the evaluation method, modeling of experimental animals suffering from traditional Chinese medicinal syndromes is easy to control, an accurate and effective way of identifying syndrome characteristics is increased, and a great convenience is brought to clinical diagnosis, therapeutic effect evaluation, novel medicine development and international communication of the traditional Chinese medicine.

Description

technical field [0001] The invention relates to the technical field of life sciences, in particular to an evaluation method for a rat model of endometriosis with kidney deficiency and blood stasis syndrome. Background technique [0002] As an indispensable means for the development of modern life sciences, animal experiments have widely penetrated into various fields of basic medicine and clinical medicine of traditional Chinese medicine. The development of TCM animal models is based on the overall concept of TCM and the thinking of syndrome differentiation and treatment, using the theory of Zangxiang and etiology and pathogenesis to simulate some characteristics of human disease prototypes in animals. The replication of standardized TCM animal models is an important link in promoting the modernization of TCM and is of great significance to the development of TCM. [0003] However, the main problems at present are: it is difficult to control the experimental animal models o...

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Problems solved by technology

[0003] However, the main problems at present are: it is difficult to control the experimental animal models of TCM syndromes, or directly use the method of disease modeling, and lack of accurate and effective ways to identify the characteristics of syndromes. Difficulties in communication between

Due to ethical and other reasons, neither controlled studies nor injury studies can be conducted on humans, and the whole process of the disease cannot be observed in EMs patients

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