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A method of measuring glycated protein

A protein and glycosylation technology, applied in the biological field, can solve the problems of increasing the difficulty and complexity of operations, increasing the operating steps of inspectors, and prolonging the testing time, etc., to achieve convenient and rapid testing process, simplify the testing operation process, and easy and fast operation Effect

Active Publication Date: 2022-04-12
无锡博慧斯生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This technology needs to add two buffer solutions with different pH values ​​during the test process. In the actual test process, adding two different buffer solutions increases the operating steps of the inspectors, increases the difficulty and complexity of the operation, and prolongs the test time. , and brings an additional risk of operator error, such as the inspector accidentally adding the buffers in the wrong order, that is, adding the second buffer first and then the first buffer, resulting in test errors

Method used

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  • A method of measuring glycated protein
  • A method of measuring glycated protein
  • A method of measuring glycated protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1 preparation device

[0050] The device prepared by the present invention is a test paper for glycated hemoglobin, and the specific structure is as follows: figure 1 shown;

[0051] The glycosylated hemoglobin test paper consists of a bottom card (PET Card), a chromatographic membrane (membrane), a water-absorbing paper (Sink) and a reflector (reflector); there is a round hole on the bottom card facing the reflective film, as a detection optics The detection hole of the reflection value; the chromatographic membrane is combined with negatively charged groups such as carboxyl and boric acid groups in the solution environment of pH 5-7; the characteristics of the chromatographic membrane are described in the patent "Used to quantify glycosylation The protein method and device" (Patent No.: CN02804372) has been described in detail.

[0052] The measurement method of the present invention is jointly completed by the glycated hemoglobin detector and the glycate...

Embodiment 2

[0054] The method provided by the present invention is used to detect glycated protein. A buffer solution is used. The allowable pH range of the buffer solution is 7-10, preferably between 8-8.5. In this buffer solution, the glycosylated hemoglobin in the blood sample Can be combined with the boronic acid group on the chromatographic membrane, and the non-glycosylated hemoglobin will be eluted along with the buffer along the chromatographic membrane; the content of total hemoglobin in the blood sample is related to the lowest value that the reflectance curve can achieve , so the total hemoglobin concentration can be obtained by measuring the minimum value; and the reflectance value will eventually stabilize at a certain level, which is related to the concentration of glycated hemoglobin in the blood sample. The higher the concentration of glycated hemoglobin in the blood sample, the reflectance will be stable at a lower value, the lower the concentration of glycosylated hemoglo...

Embodiment 3

[0059] Choose two blood samples, the values ​​of glycosylated hemoglobin are 5.2% and 11.6% respectively, utilize the one-step method of embodiment 2 to test 6 times respectively, the buffer solution that test uses is 50mM Tris-HCl, 100mM NaCl, 1%Tween 20, pH is 8.5, the result obtained is:

[0060] See Table 1 and Table 2 for the test values ​​of the blood sample with a glycosylated hemoglobin value of 5.2%.

[0061] Table 1 Test value of blood sample with glycosylated hemoglobin value of 5.2%

[0062] 2-Wet 3-THb 4-GHb Conc-THb Conc-GHb Ratio 1 77.92 24.16 59.22 1.038 0.065 0.0626 2 78.53 24.74 58.90 1.031 0.066 0.0640 3 77.34 26.30 59.17 1.005 0.065 0.0647 4 77.75 24.88 58.00 1.029 0.068 0.0661 5 76.94 23.77 60.01 1.044 0.062 0.0594 6 77.92 24.94 59.22 1.026 0.065 0.0634

[0063] Table 2 Test values ​​of blood samples with glycosylated hemoglobin of 11.6%

[0064] 2-Wet 3-T...

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Abstract

The present invention provides a method for measuring glycosylated protein. The method is based on the principle that hydroxyboryl compounds bind to glycosylated protein but not to non-glycosylated protein. The method is improved on the prior art, and only one buffer solution is used to obtain The percentage of glycosylated hemoglobin in the blood sample can significantly simplify the test operation process, making the test process convenient and fast, and making it possible for diabetic patients who have not received special training to test glycosylated hemoglobin at home, which has broad application prospects and huge potential. Market value.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for measuring glycated protein. Background technique [0002] According to the latest epidemiological survey, diabetes has 370 million patients worldwide and 110 million patients in China. The market is huge and growing rapidly. Moreover, according to research, only 1 / 3 of diabetics in my country have been diagnosed in time, and only 1 / 3 of all diagnosed patients have received effective treatment, so that blood sugar has been well controlled. Diabetes poses a huge threat to the health of the public in our country and brings a heavy burden to medical insurance expenditure. At present, the medical expenses of diabetes and its complications have accounted for about 20% of the total medical expenditure. [0003] Proteins in blood, including albumin in plasma, hemoglobin in red blood cells, etc., will slowly undergo non-enzymatic reactions with glucose in blood to form glycated pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/55G01N33/68C07K14/805C07K1/22
CPCG01N21/55G01N33/6893C07K14/805G01N2800/042
Inventor 彭伟李俊谭中邹丽洁林滔
Owner 无锡博慧斯生物医药科技有限公司
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