Pseudo-planting matrix beneficial for growth of tissue cultured seedlings of bletilla striata and preparation method thereof
A technique for tissue culture seedlings and substrates, applied in the field of plant tissue culture, can solve the problems of restricting the application of white chia tissue culture seedlings, increasing the cost of seedlings, slow growth, etc., and achieving the effects of easy operation, saving manpower and material resources, and less investment.
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Embodiment 1
[0019] This embodiment illustrates the influence of degreasing pine bark, river sand, and loess single substrate on artificial planting of white and tissue culture seedlings through comparative experiments.
[0020] The preparation method of the pseudo-plant matrix of the growth of white and tissue culture seedlings comprises the following steps:
[0021] (1) Substrate preparation: The defatted pine bark is granular, about 0.7 cm in size; put the defatted pine bark in a woven bag, put it in a pool, press it with stones, pour water into the pool, submerge the woven bag, soak During 2 days, the water level dropped, so it should be filled up in time to fully soak the skim pine bark; the river sand was semi-dry and wet; the loess was excavated from the mountain and no crops had been planted;
[0022] (2) Substrate laying: In the greenhouse, use degreased pine bark, river sand, and loess to make furrows respectively. The length, width, and height of the furrows are 2.6 m×1.2 m×16 c...
Embodiment 2
[0031] This embodiment is an experiment using a combination of any two substrates in defatted pine bark, river sand, and loess to affect the artificial planting of white and tissue culture seedlings. The first is defatted pine bark+river sand, and the second is defatted pine bark + loess, the third type is river sand + loess.
[0032] The preparation method of the pseudo-plant matrix of the growth of white and tissue culture seedlings comprises the following steps:
[0033] (1) Substrate preparation: The defatted pine bark is granular, about 0.7 cm in size; put the defatted pine bark in a woven bag, put it in a pool, press it with stones, pour water into the pool, submerge the woven bag, soak During 2 days, the water level dropped, so it should be filled up in time to fully soak the skim pine bark; the river sand was semi-dry and wet; the loess was excavated from the mountain and no crops had been planted;
[0034] (2) Substrate laying: In the greenhouse, the first substrate ...
Embodiment 3
[0043] This embodiment is to test defatted pine bark, river sand, and loess three kinds of substrates when using simultaneously, the impact of different heights of defatted pine bark layer on white and tissue culture seedling false planting.
[0044] A preparation method for a pseudo-plant matrix that is beneficial to the growth of white and tissue-cultured seedlings, comprising the following steps:
[0045] (1) Substrate preparation: The defatted pine bark is granular, about 0.7 cm in size; put the defatted pine bark in a woven bag, put it in a pool, press it with stones, pour water into the pool, submerge the woven bag, soak During 2 days, the water level dropped, so it should be filled up in time to fully soak the skim pine bark; the river sand was semi-dry and wet; the loess was excavated from the mountain and no crops had been planted;
[0046] (2) Substrate laying: In the greenhouse, use loess to build a bed. The length × width × height of the bed is 2.6 m × 1.2 m × 6 cm...
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