Pseudo-planting matrix beneficial for growth of tissue cultured seedlings of bletilla striata and preparation method thereof

A technique for tissue culture seedlings and substrates, applied in the field of plant tissue culture, can solve the problems of restricting the application of white chia tissue culture seedlings, increasing the cost of seedlings, slow growth, etc., and achieving the effects of easy operation, saving manpower and material resources, and less investment.

Active Publication Date: 2019-03-22
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are problems such as slow growth and low survival rate when directly used for field planting, resulting in increased cost of seedlings, which greatly limits the application of white and tissue cultured seedlings.

Method used

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  • Pseudo-planting matrix beneficial for growth of tissue cultured seedlings of bletilla striata and preparation method thereof
  • Pseudo-planting matrix beneficial for growth of tissue cultured seedlings of bletilla striata and preparation method thereof
  • Pseudo-planting matrix beneficial for growth of tissue cultured seedlings of bletilla striata and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] This embodiment illustrates the influence of degreasing pine bark, river sand, and loess single substrate on artificial planting of white and tissue culture seedlings through comparative experiments.

[0020] The preparation method of the pseudo-plant matrix of the growth of white and tissue culture seedlings comprises the following steps:

[0021] (1) Substrate preparation: The defatted pine bark is granular, about 0.7 cm in size; put the defatted pine bark in a woven bag, put it in a pool, press it with stones, pour water into the pool, submerge the woven bag, soak During 2 days, the water level dropped, so it should be filled up in time to fully soak the skim pine bark; the river sand was semi-dry and wet; the loess was excavated from the mountain and no crops had been planted;

[0022] (2) Substrate laying: In the greenhouse, use degreased pine bark, river sand, and loess to make furrows respectively. The length, width, and height of the furrows are 2.6 m×1.2 m×16 c...

Embodiment 2

[0031] This embodiment is an experiment using a combination of any two substrates in defatted pine bark, river sand, and loess to affect the artificial planting of white and tissue culture seedlings. The first is defatted pine bark+river sand, and the second is defatted pine bark + loess, the third type is river sand + loess.

[0032] The preparation method of the pseudo-plant matrix of the growth of white and tissue culture seedlings comprises the following steps:

[0033] (1) Substrate preparation: The defatted pine bark is granular, about 0.7 cm in size; put the defatted pine bark in a woven bag, put it in a pool, press it with stones, pour water into the pool, submerge the woven bag, soak During 2 days, the water level dropped, so it should be filled up in time to fully soak the skim pine bark; the river sand was semi-dry and wet; the loess was excavated from the mountain and no crops had been planted;

[0034] (2) Substrate laying: In the greenhouse, the first substrate ...

Embodiment 3

[0043] This embodiment is to test defatted pine bark, river sand, and loess three kinds of substrates when using simultaneously, the impact of different heights of defatted pine bark layer on white and tissue culture seedling false planting.

[0044] A preparation method for a pseudo-plant matrix that is beneficial to the growth of white and tissue-cultured seedlings, comprising the following steps:

[0045] (1) Substrate preparation: The defatted pine bark is granular, about 0.7 cm in size; put the defatted pine bark in a woven bag, put it in a pool, press it with stones, pour water into the pool, submerge the woven bag, soak During 2 days, the water level dropped, so it should be filled up in time to fully soak the skim pine bark; the river sand was semi-dry and wet; the loess was excavated from the mountain and no crops had been planted;

[0046] (2) Substrate laying: In the greenhouse, use loess to build a bed. The length × width × height of the bed is 2.6 m × 1.2 m × 6 cm...

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Abstract

The invention discloses a pseudo-planting matrix beneficial for growth of tissue cultured seedlings of bletilla striata and a preparation method thereof. The pseudo-planting matrix is divided into three layers, the upper layer is defatted pine bark, the middle layer is river sand and the lower layer is loess. The method comprises the steps: during preparation, the defatted pine bark is packed in awater permeable bag, placed in a pond and pressed with heavy objects, water is irrigated into the pond, the water permeable bag is immersed, soaking is performed for 2 d, the water level drops duringsoaking, the pond is full of water in time, and the defatted pine bark can be fully soaked thoroughly; the humidity of the river sand is 40%-60%; loess uncultivating crops is adopted; in a greenhouse, bedding is performed with the loess, the length of the bed is the same as that of the greenhouse, and the width and the height of the bed are 1.2 m and 6 cm respectively; a layer of river sand is laid on the loess, and the height is 6 cm; a layer of defatted pine bark soaked in water is laid on the river sand, and the height is 4 cm; the total height of the bed is 16 cm; and the matrix is used after being laid immediately. The preparation method of the pseudo-planting matrix is simple and easy to operate; not only are the needs of ventilation and humidity of bletilla striata as root systemsof orchidaceae satisfied, but also a seedbed also can be reused.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a pseudo-plant matrix and a preparation method thereof which are beneficial to the growth of tissue culture seedlings. Background technique [0002] White and ( Bletilla striata (Thunb.) Reichb.f.) is a perennial herb of Orchidaceae, with large and colorful flowers and high ornamental value; its pseudobulb is a traditional Chinese medicine in my country, which was first recorded in "Shen Nong's Materia Medica" and included in "Pharmacopoeia of the People's Republic of China" , and has been included in the traditional Chinese medicine for both medicine and food. Baiji has the effects of astringent, hemostasis, detumescence and granulation. It is used for hemoptysis, hematemesis, traumatic bleeding, sore swelling, and chapped skin. The main ingredient of Chinese patent medicines such as "Yunnan Baiyao". [0003] Baiji is distributed in most parts of my country, including areas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G24/22A01G24/10A01G22/00A01G22/60
CPCA01G22/00A01G22/60A01G24/10A01G24/22
Inventor 石云平林茜苏祖祥许娟韦绍龙李小泉
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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