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A liquid compound lactobacillus preparation and its preparation method and application

A technology of compounding Lactobacillus and Lactobacillus, applied in the field of feed additives, can solve the problems of complex production process, reduced number of viable bacteria, short storage period, etc., and achieve the effects of rich metabolites, high content of viable bacteria and low cost

Active Publication Date: 2021-03-09
FUJIAN AONONG BIOLOGICAL TECH GRP CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, solid preparations have complex manufacturing processes, and the number of viable bacteria will decrease after storage, processing and mixing with complete materials.
The preparation process of liquid lactic acid bacteria preparation is simple and convenient to use, but its disadvantage is that it is inconvenient to transport and has a short storage period at room temperature.

Method used

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  • A liquid compound lactobacillus preparation and its preparation method and application
  • A liquid compound lactobacillus preparation and its preparation method and application
  • A liquid compound lactobacillus preparation and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Prepare lactic acid bacteria liquid culture medium according to the formula in Table 1 below:

[0029] Table 1 liquid medium formula

[0030]

[0031] Inoculate the preserved Lactobacillus plantarum, Lactobacillus acidophilus and Bacillus bulgaricus strains into 500mL liquid medium respectively, and culture them statically at 37°C for 24 hours, and mix the three bacterial solutions after static fermentation to prepare mixed Lactobacillus seeds Liquid 1500mL.

[0032] 2. Put the mixed Lactobacillus seed solution into a 1t fermentation tank for fermentation, and use the conventional fermentation process to ferment the Lactobacillus. The fermentation process conditions are: temperature 37°C, pH=7.2, speed 80-100r / min, fermentation 18- 24h. The fermentation medium formula is as shown in table 2:

[0033] Table 2 Fermentation Medium Formula

[0034]

[0035]

[0036] 3. Prepare the protective agent according to the following formula: 1-3 parts of glucose, 0.0...

Embodiment 2

[0053] A liquid compound lactobacillus preparation comprises the following components and contents in parts by weight: 3 parts of compound lactobacilli, 15 parts of a protective agent, and the pH value of the preparation is 3.9.

[0054] The preparation method of this liquid compound lactobacillus preparation comprises the following steps:

[0055] 1) Inoculate the compound lactobacillus on the liquid medium, and culture it statically at 40°C for 20 hours to obtain the mixed lactobacillus seed liquid;

[0056] 2) Inoculate the mixed Lactobacillus seed liquid on the fermentation medium, and ferment at 40°C for 18 hours. During the fermentation process, the pH value is 7.5, and the stirring speed is 80r / min to obtain the fermentation liquid;

[0057] 3) Centrifuge the fermentation bacteria liquid. During the centrifugation process, the centrifuge speed is 4500r / min, and the centrifugation time is 4min; After being uniform, a liquid compound lactobacillus preparation is obtained...

Embodiment 3

[0060] A liquid compound lactobacillus preparation comprises the following components and contents in parts by weight: 4.5 parts of compound lactobacilli, 9 parts of a protective agent, and the pH value of the preparation is 5.2.

[0061] The preparation method of this liquid compound lactobacillus preparation comprises the following steps:

[0062] 1) Inoculate the compound lactobacillus on the liquid medium, and culture it statically at 35°C for 28 hours to obtain the mixed lactobacillus seed liquid;

[0063] 2) Inoculate the mixed Lactobacillus seed liquid on the fermentation medium, and ferment at 35°C for 24 hours. During the fermentation process, the pH value is 7.0, and the stirring speed is 100r / min to obtain the fermentation liquid;

[0064] 3) Centrifuge the fermentation bacteria liquid. During the centrifugation process, the centrifuge speed is 3500r / min, and the centrifugation time is 6min; After being uniform, a liquid compound lactobacillus preparation is obtain...

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Abstract

The invention relates to a liquid compound lactobacillus preparation and its preparation method and application. The preparation comprises the following components and contents in parts by weight: 3-4.5 parts of compound lactobacillus, 9-15 parts of protective agent, and the pH value of the preparation is 3.9-5.2 When preparing, first prepare the mixed lactobacillus seed liquid, then prepare the fermented bacterial liquid, and finally add the bacterial slime to the protective agent and mix evenly. Compared with the prior art, the present invention aims at the defect that the shelf life of the liquid lactic acid bacteria preparation is relatively short, by adding a protective agent containing energy substances in the liquid compound lactic acid bacteria preparation for the survival of the bacteria, and maintaining a lower pH to delay The growth rate of the bacteria can be increased, and finally the purpose of prolonging the shelf life of the liquid compound lactobacillus preparation at room temperature can be achieved, thereby improving its practical application effect in pig farms.

Description

technical field [0001] The invention belongs to the technical field of feed additives, and relates to a liquid compound lactobacillus preparation and a preparation method and application thereof. Background technique [0002] At present, substances such as antibiotics and chemical drugs have been widely used in conventional farming, which has destroyed the balance of intestinal flora in animals and reduced the animal's own immunity. At the same time, antibiotic resistance and drug residues in animal products have attracted increasing attention. Finding safe and green additives to replace antibiotics is an urgent problem in animal husbandry production. [0003] Probiotics have been paid attention to and utilized because of their advantages of no pollution, no residue, safety and reliability, and are ideal substitutes for antibiotics. Among the many microbial additives, lactic acid bacteria preparations are the most widely used and effective type of microbial additives for fe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/18A23K50/30C12R1/25C12R1/23C12R1/225
CPCA23K10/18A23K50/30C12N1/20
Inventor 张诗赖水明张志榕兰玉华刘小成张敬学仲伟迎傅心锋梁世仁吴有林
Owner FUJIAN AONONG BIOLOGICAL TECH GRP CO LTD
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