Method for increasing induction frequency of new inter-subspecific tetraploid rice germplasm

A new germplasm and tetraploid technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problem of low induction efficiency

Inactive Publication Date: 2019-03-19
黄群策
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Problems solved by technology

[0005] The present invention proposes a method for increasing the frequency of new germplasm induction of tetraploid rice among subspecies, so as to solve the technical problem of low induction efficiency of existing methods

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  • Method for increasing induction frequency of new inter-subspecific tetraploid rice germplasm

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Embodiment Construction

[0023] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0024] like figure 1 As shown, the diploid indica rice variety “IR36(2)”, japonica rice variety “Xindao 10(2)” and their hybrid F1 and hybrid F2 were used as materials to induce tetraploid rice between indica and japonica subspecies. The testing of the germplasm can be summarized as an example of the inventive technique. In this research system, sexual crossing was carried out with "IR36(2)" and "Xindao 10(2)" as the hybrid parents, and the first generation (...

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Abstract

The invention proposes a method for increasing induction frequency of new inter-subspecific tetraploid rice germplasm. The method comprises the steps of by means of induction technique of rice genomepolyploidization, firstly obtaining an inter-subspecific hybrid population (F1) with a heterozygous genetic basis through sexual hybridization among cultivated rice subspecies; obtaining a heterozygous population (F2) with various genotypes through self-crossing; and inducing and creating the new inter-subspecific tetraploid rice germplasm, thereby improving the induction efficiency of the new inter-subspecific tetraploid rice germplasm. In a same experiment, if the concentration (0.1%) of the inducing agent and the induction time (72h) are specific values and the adopted experimental materialis of a heterozygous genotype, i.e., a genetic heterozygote, the probability of successfully inducing the genome polyploidization is relatively big, that is, in a same induction experiment, when thegenome polyploidization induction is simultaneously carried out on various genotype materials, the probability of successful induction is greatly increased.

Description

technical field [0001] The invention relates to a method for increasing the induction frequency of new germplasm of tetraploid rice among subspecies. Background technique [0002] In the early 1950s, researchers opened the prelude to the study of polyploid rice in my country. Due to many objective and subjective reasons, the current research on polyploid rice has not achieved the research goals expected by researchers, and its potential value has not attracted the attention of most rice researchers. The limitation of the number of materials (or material types) in the study of autopolyploid is mainly reflected in the fact that after more than 60 years of induction and research, the number of autopolyploid rice materials we have obtained has not exceeded 2,000. The ecotypes or genotypes are rather limited. It can be seen that there are still some technical limitations in the induction technology and induction method for obtaining new germplasm of autotetraploid rice, and the...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 黄群策
Owner 黄群策
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