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Use of thermolysin to reduce or eliminate bacterial biofilms from surfaces

A technology of thermolysin and bacterial biofilm, applied in antibacterial drugs, peptide/protein components, biochemical equipment and methods, etc.

Pending Publication Date: 2019-03-15
SMITH & NEPHEW INC
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, many bacteria are becoming increasingly resistant to these compounds

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  • Use of thermolysin to reduce or eliminate bacterial biofilms from surfaces

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example 1

[0059] Example 1: In vitro biofilm studies with thermolysin and collagenase

[0060] In vitro assays were performed to demonstrate the bacterial biofilm reducing capacity of thermolysin. In this assay, S. aureus ATCC 6538 was suspended in growth medium of tryptic soy broth supplemented with 0.25% glucose for optimal bacterial biofilm formation. The suspension was transferred to the wells of a sterile 96-well culture plate and incubated at 37°C for 22 hours with one medium change. After the bacterial biofilm was formed, the growth medium was replaced with a thermolysin solution dissolved in the growth medium at different concentrations and a collagenase solution dissolved in the growth medium at the same concentration (enzyme concentration was 0.000001 mg / mL, 0.00001mg / mL, 0.0001mg / mL, 0.001mg / mL, 0.01mg / mL, 0.1mg / mL, 1.0mg / mL and 10mg / mL). After 16 hours, the remaining attached bacteria were quantified by aspirating the enzyme solution (enzyme + growth medium) and washing th...

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Abstract

Methods are disclosed for the reduction or elimination of bacterial biofilms on biological and non-biological surfaces, as well as methods for the treatment of wounds, skin lesions, mucous membrane lesions, and other biological surfaces infected or contaminated with bacterial biofilms using compositions comprising thermolysin.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of U.S. Provisional Patent Application Serial No. 62 / 367,338, filed July 27, 2016. The entire contents of the cited applications are incorporated herein by reference. technical field [0003] The present invention generally relates to methods and compositions for reducing or eliminating bacterial biofilms on surfaces. The composition includes thermolysin as an active ingredient to reduce or eliminate bacterial biofilm. Background technique [0004] A bacterial biofilm is a population of bacteria attached to a surface. Bacteria in biofilms are typically embedded in an autogenous matrix of extracellular polymeric substances (EPS) that holds bacteria together in clumps and firmly attaches the clumps to the underlying surface. Bacterial biofilm EPS, commonly referred to as slime, are polymeric aggregates typically composed of extracellular DNA, proteins, polysaccharides, and various ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/48A61L31/08A61K9/00A61P31/04
CPCA61K38/4886C12Y304/24027A61L27/34A61L27/54A61L29/085A61L29/16A61L31/10A61L31/16A61L2300/254A61P17/02A61P31/04A01N25/34A01N33/18
Inventor 石磊凯瑟琳·范德卡尔阿列克萨·约万诺维奇埃里克·罗奇
Owner SMITH & NEPHEW INC
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