Application of nano zinc oxide in improving salt stress tolerance of plants
A nano-zinc oxide, plant technology, applied in the application, plant growth regulator, plant growth regulator and other directions, can solve the problems such as no report of the application of nano-zinc oxide.
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Embodiment 1
[0025] The application of nano-zinc oxide in improving the resistance of plants to salt stress comprises the following steps:
[0026] 1. Wild-type Arabidopsis Seedling Preparation
[0027] Select wild-type Arabidopsis thaliana seeds with the same size and plump grains at the same period, disinfect them with 70% ethanol aqueous solution and 1.25% sodium hypochlorite aqueous solution for 5 minutes and 10 minutes, rinse with sterile water, remove the water, and put Put it into a test tube, add sterile water, submerge the seeds, and place them in the dark at 4°C for 3 days to vernalize the seeds and restore their vitality.
[0028] Spread the vernalized seeds on 1 / 2MS solid medium in a single row, wrap the petri dish with sterile air-permeable tape, mark the date, and place the petri dish vertically in the growth chamber under constant 16h light / 8h dark state Next, the light intensity was set at 120 μmolm -2s-1 , grown for three days to obtain seedlings.
[0029] 2. Seedling t...
Embodiment 2
[0043] The application of nano-zinc oxide in improving the resistance of plants to salt stress comprises the following steps: irrigate wild-type Arabidopsis planted in saline soil with a nano-zinc oxide aqueous suspension with a concentration of 10 mg / L.
Embodiment 3
[0045] The application of nano-zinc oxide in improving the resistance of plants to salt stress comprises the following steps: irrigate the wild-type Arabidopsis planted in saline soil with a nano-zinc oxide aqueous suspension with a concentration of 35mg / L.
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