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Intestinal flora capsule preparation method and intestine flora capsules

A technology of capsule preparation and enterobacteria capsule, which is applied in the field of microbiology, can solve the problems of long treatment cycle and achieve the effects of shortening the treatment cycle, increasing colon motility, and relieving pain

Inactive Publication Date: 2019-03-01
SHANGHAI TENTH PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This treatment method has a certain effect, but the treatment cycle is long, and a single probiotic cannot maintain a good balance of intestinal flora

Method used

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  • Intestinal flora capsule preparation method and intestine flora capsules
  • Intestinal flora capsule preparation method and intestine flora capsules

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Experimental program
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Effect test

Embodiment 1

[0050] The invention provides a preparation method of enterobacteria capsule, comprising the following steps:

[0051] Step S1, obtaining human feces, mixing human feces with sterile saline in a certain proportion to obtain a mixed solution;

[0052] Step S2, stirring the mixed solution to transform the mixed solution into a suspension;

[0053] Step S3, performing microfiltration on the suspension to obtain a filtrate;

[0054] Step S4, centrifuging the filtrate to obtain a precipitate;

[0055] Step S5, mixing the precipitate with the lyoprotectant in a certain proportion to obtain a pre-freeze solution, pre-freezing the pre-freeze solution and performing freeze-drying to obtain a lyophilized powder;

[0056] Step S6, adding the freeze-dried powder to the capsule, sealing and packaging to obtain the Enterobacteria capsule, and storing the Enterobacteria capsule at -20°C.

[0057] In the present invention, the active ingredient of the enteric bacteria capsule is sediment, ...

Embodiment 2

[0111] This embodiment is a specific embodiment of the present invention.

[0112] A preparation method of enterobacteria capsule, comprising the following steps:

[0113] Step S1. Obtain 100g of human feces, put it into a sterile mixing tank, pour 300ml of 0.9% sterile saline, and stir to obtain a mixed solution;

[0114] Step S2, stirring the mixed solution, so that the flora in human feces is released from the feces, so that the mixed solution becomes a suspension rich in flora;

[0115] Step S3, the suspension can be subjected to three-stage microfiltration treatment, so that the suspension passes through filter screens with apertures of 2.0mm, 0.5mm, and 0.2mm in sequence to obtain the filtrate from which residues are removed;

[0116] Step S4, perform low-temperature centrifugation on the filtrate, the working conditions are: temperature 4°C, rotation speed 2000rpm, centrifugation time 3min, remove the supernatant to obtain a precipitate, which is the enterobacteria com...

Embodiment 3

[0120] This embodiment is a specific embodiment of the present invention.

[0121] A preparation method of enterobacteria capsule, comprising the following steps:

[0122] Step S1: Obtain 100g of human feces, put it into a sterile mixing tank, pour 500ml of 0.9% sterile saline, stir and mix to obtain a mixed solution;

[0123] Step S2, stirring the mixed solution, so that the flora in human feces is released from the feces, so that the mixed solution becomes a suspension rich in flora;

[0124] Step S3, the suspension can be subjected to secondary microfiltration treatment, so that the suspension passes through filter screens with apertures of 2.0 mm and 0.5 mm respectively, to obtain a filtrate from which residues are removed;

[0125] Step S4, perform low-temperature centrifugation on the filtrate, the working conditions are: temperature 4°C, rotation speed 2500rpm, centrifugation time 5min, remove the supernatant to obtain a precipitate, which is the enterobacteria composi...

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Abstract

The invention relates to an intestinal flora capsule preparation method. The preparation method comprises the following steps: S1, collecting human excrement, and mixing the human excrement and sterile saline solution according to a certain proportion to obtain a mixed solution; S2, stirring the mixed solution to transform the mixed solution into a suspension; S3, performing micro-filtering treatment on the suspension to obtain filtrate; S4, centrifuging the filtrate to obtain a precipitate; S5, mixing the precipitate and a freeze-drying protective additive according to a certain proportion toobtain a pre-frozen solution, and performing freeze drying after the pre-frozen solution is pre-frozen to obtain freeze-dried powder; S6, adding the freeze-dried powder into capsules, sealing and packaging to obtain the intestinal flora capsules, and storing the intestinal flora capsules in an environment at a temperature of 20 DEG C below zero. The invention further relates to the intestinal bacterial capsules. The intestinal bacterial capsules have the advantages of containing a compound of probiotics, bifidobacterial, lactobacillus, aerobic bacteria, escherichia coli and anaerobic bacteria, being capable of remarkably regulating the intestinal flora balance and shortening the treatment period.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a method for preparing enterobacteria capsules and enterobacteria capsules. Background technique [0002] Chronic constipation is a common intestinal disease. In my country, the prevalence of chronic constipation in adults is 4% to 6%, and it increases with age. The prevalence of chronic constipation in people over 60 years old is as high as 22%. [0003] The existing method for the treatment of chronic constipation is the use of traditional medicines, such as laxatives or laxatives. However, this treatment method has certain side effects, and the therapeutic effect is limited, so it cannot effectively alleviate the patient's pain. [0004] In addition to the use of traditional medicines, in recent years, a treatment for constipation by improving the gut microbiota has emerged. This treatment method usually uses probiotics to adjust the intestinal flora of patients to improve ...

Claims

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Application Information

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IPC IPC(8): A61K35/747A61K9/48A61P1/10A61K35/741A61K35/745A61K35/74
CPCA61K9/4875A61K35/74A61K35/741A61K35/745A61K35/747A61P1/10A61K2300/00Y02A50/30
Inventor 田宏亮李宁马春联陈启仪赵笛
Owner SHANGHAI TENTH PEOPLES HOSPITAL
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