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Rhizosphere soil culture device and method

A technology of cultivation device and cultivation method, which is applied in the direction of sampling device, horticultural method, botany equipment and method, etc., and can solve problems such as inability to effectively obtain gradient rhizosphere soil and difficulty in obtaining rhizosphere soil

Pending Publication Date: 2019-03-01
衢州市农业科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a rhizosphere soil culture device and method in order to overcome the difficulty in obtaining rhizosphere soil in the prior art and the problem that gradient rhizosphere soil cannot be effectively obtained

Method used

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  • Rhizosphere soil culture device and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0051] a) Use the weighing method to select a plurality of corn seeds with the same weight, and use a concentration of 10% by weight of H 2 o 2 Soak for 20 minutes for disinfection, then rinse repeatedly with deionized water until clean;

[0052] b) Wash the clean seeds obtained in step a) with saturated CaSO 4 Soak in the solution for 10 hours, and protect from light for 12 hours of ventilation and swelling; then keep it moist for 24 hours to accelerate germination at a constant temperature and avoid light;

[0053] c) the germinated seeds with 1cm root system obtained in step b) are carried out rolling seed, and the rolling seed method is that the germinated seeds are placed in the tubular filter paper, and the root system direction of the germinated seeds is parallel to the axis direction of the tubular filter paper; The cylindrical filter paper with germinated seeds is placed vertically, so that the end of the cylindrical filter paper is soaked in the culture solution, a...

Embodiment 2

[0056] a) Use the weighing method to select a plurality of corn seeds with the same weight, and use a concentration of 10% by weight of H 2 o 2 Soak for 30 minutes for disinfection, then rinse repeatedly with deionized water until clean;

[0057] b) Wash the clean seeds obtained in step a) with saturated CaSO 4 Soak in the solution for 12 hours, and avoid light, ventilate and inflate for 20 hours; then keep it moist for 35 hours at a constant temperature and avoid light;

[0058] c) the germinated seeds with 3cm root system obtained in step b) are carried out rolling seed, and the rolling seed method is that the germinated seeds are placed in the tubular filter paper, and the root system direction of the germinated seeds is parallel to the axial direction of the tubular filter paper; The cylindrical filter paper with germinated seeds is placed vertically, and the end of the cylindrical filter paper is soaked in the culture solution, and the plant root system grows to 20cm in...

Embodiment 3

[0061] a) Use the weighing method to select a plurality of corn seeds with the same weight, and use a concentration of 10% by weight of H 2 o 2 Soak for 25 minutes for disinfection, then rinse repeatedly with deionized water until clean;

[0062] b) Wash the clean seeds obtained in step a) with saturated CaSO 4 The solution was soaked for 11 hours, and protected from light and ventilated for 15 hours; then kept moist for 30 hours at a constant temperature and protected from light;

[0063] c) the germinated seeds with 2cm root system obtained in step b) are carried out rolling seed, and the rolling seed method is that the germinated seeds are placed in the tubular filter paper, and the root system direction of the germinated seeds is parallel to the axial direction of the tubular filter paper; The tube-shaped filter paper with germinated seeds is placed vertically, and the end of the tube-shaped filter paper is soaked in the culture solution, and the plant root system grows ...

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Abstract

The invention relates to the field of plant rhizosphere soil research equipment, and particularly relates to a rhizosphere soil culture device and method. The rhizosphere soil culture device comprisesa shell for containing soil and a plant root system; the top surface of the shell is open; a position, close to a side surface A, of the bottom surface of the shell is provided with a gap for enabling the plant root system to grow downwards into a culture solution tank; the rhizosphere soil culture device further comprises a water-permeable thin film which cannot be penetrated by the plant root system; and the water permeable thin film is arranged in the shell and divides the inner space of the shell into a soil containing part and a plant root system containing part; The invention further discloses a rhizosphere soil culture method suitable for the device. Through the use of the device and the method, a large amount of complete rhizosphere soil can be obtained, and meanwhile, the obtained rhizosphere soil can show a gradient effect of the plant root system on the soil.

Description

technical field [0001] The invention relates to the field of plant rhizosphere soil cultivation, in particular to a rhizosphere soil cultivation device and method. Background technique [0002] At present, the collection of rhizosphere exudates is mainly concentrated in hydroponics, and there are also matrix culture collection methods and soil culture methods. The rhizosphere environment and plant physiological conditions are quite different between the hydroponics method and the matrix cultivation method and the soil cultivation conditions in which the plants actually grow. The traditional soil culture method is to take out the root system of the plant and rinse it with distilled water, and use the rinse solution as the rhizosphere exudate. During the whole operation process, the root system is very vulnerable to damage, and the contents and wound fluid of the root system itself will damage the plant itself. root exudates. According to different research purposes and rese...

Claims

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Application Information

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IPC IPC(8): A01G9/02A01C1/00A01C1/08A01G7/00G01N1/04
CPCA01C1/00A01C1/08A01G7/00A01G9/02G01N1/04
Inventor 石子建李海港李建辉
Owner 衢州市农业科学研究院
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