Rapid detection method and detection kit for trimethylamine oxide in urine

Abstract

The invention discloses a rapid detection method for trimethylamine oxide in urine, wherein the method comprises the following steps: regulating the pH of urine, adding Reinecke salt, shaking evenly,then carrying out a reaction, and determining the content of TMAO in the urine by colorimetric analysis or absorption photometry. Before the pH value of the urine is regulated, the method can also include the step of pretreatment of the urine with quicklime; previously, before pretreatment, the pH value of the urine is tested, and if the pH value is less than 7, the pH value of the urine is regulated to 7 or more. The kit comprises a pH regulator, a chromogenic reagent and a pretreatment reagent. The pretreatment reagent contains quicklime, and the chromogenic reagent contains Reinecke salt. The method can realize rapid and accurate determination of the concentration of TMAO in solutions.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to a rapid detection method and a detection kit for trimethylamine oxide in urine. Background technique [0002] Trimethylamine oxide (Trimetlylamine oxide, TMAO) is a metabolite produced by choline, betaine and carnitine, and is also a key biomarker of human heart or kidney disease. Recent studies have shown that it can also be used for colorectal Auxiliary diagnostic indicators for cancer. TMAO exerts cardiovascular effects by affecting the metabolism of cholesterol in human organs, and is a potential risk indicator for stroke, arteriosclerosis, coronary artery disease and heart failure. Recently, it has been reported that there is also a direct correlation between fasting blood TMAO levels and the long-term risk of various cardiovascular diseases. The higher the concentration of TMAO in the body (for example, higher than 5mg / L), the greater the risk of cardiovascular diseases....

AI Technical Summary

Problems solved by technology

However, these methods all reduce TMAO to trimethylamine (TMA) first, and then convert the content of TMAO through the amount of TMA. The experimental process is more complicated. Therefore, many reagents are required, and it is easy to cause the escape of TMA gas. , resulting in excessive deviation of the experimental results

Due to the complex operation, low detection efficiency and poor equipment portability of these methods, the scope of application is limited to a certain extent, especially not applicable to diagnostic services for rapid judgment results

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