Microfluidic drop-generating chip

Abstract

The invention discloses a microfluidic drop-generating chip, comprising a chip body, a sealing layer, sample phase injection holes and continuous phase injection holes running through the upper and lower surfaces of the chip body, a drop storage cell, and a continuous phase passage, a sample phase passage, a sample phase branch passage, a continuous phase filter region, and a sample phase filter region which are arranged on the lower surface of the chip body.

Description

technical field [0001] The invention relates to the technical field of digital PCR, in particular to a microfluidic droplet generating chip. Background technique [0002] At present, the methods for preparing droplets based on microfluidics mainly include T-channel method and fluid focusing method. The T-shaped channel method utilizes the geometric characteristics of the intersection of two microchannels to generate droplets due to the momentum change caused by the shear force of the continuous phase when the front of the continuous phase liquid turns at the intersection. The flow focusing method is to make the continuous phase fluid "squeeze" the front of the continuous phase liquid from both sides of the intersection, and use the "neck" chip of the channel downstream of the liquid front to shrink and deform the front of the dispersed liquid and destabilize it, thereby Droplets are formed. The disadvantage of these two methods is that it is necessary to adjust the pressur...

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Problems solved by technology

Generation of droplets in this manner results in a partial loss of the sample before reaching equilibrium

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