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Algae filament resuscitation fluid for shellfish feeding microalgae

A technology for resuscitation liquid and algae species, applied in the directions of microorganism-based methods, microorganisms, and unicellular algae, etc., which can solve the problems that harmful protozoa are not effectively controlled, and shellfish-feeding algae recovery has no obvious effect. The effect of improving immunity, avoiding waste of culture medium and reducing production cost

Inactive Publication Date: 2019-02-15
威海长青海洋科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the cultivation process of shellfish-eating algae, it was found that the harmful protozoa (ciliates, abdominal caterpillars, amoebas, etc.) were not effectively controlled by using the existing culture medium (medium), and at the same time, the growth of shellfish-eating algae was not effectively controlled. Recovery has no obvious effect, so how to achieve the purpose of killing harmful protozoa by adjusting the pH of the culture medium (culture medium), and at the same time, how to quickly recover and grow microalgae has become a technical problem that needs to be solved urgently

Method used

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  • Algae filament resuscitation fluid for shellfish feeding microalgae
  • Algae filament resuscitation fluid for shellfish feeding microalgae
  • Algae filament resuscitation fluid for shellfish feeding microalgae

Examples

Experimental program
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Effect test

Embodiment 1

[0025] A kind of algae resuscitating liquid for shellfish feeding on microalgae, characterized in that it comprises the following components by weight:

[0026] 500 parts of potassium nitrate;

[0027] 1000 parts of potassium dihydrogen phosphate;

[0028] 200 parts of disodium edetate;

[0029] 200 parts of ferric chloride;

[0030] 100 parts of sodium bicarbonate;

[0031] Vitamin B 1 20 copies;

[0032] Vitamin B 12 10 copies;

[0033] 5 parts of boric acid;

[0034] 2000 parts of distilled water.

[0035] The resuscitation solution was formed by mixing the above raw materials, and then HCL was added to the resuscitation solution to adjust the pH value of the resuscitation solution. Table 1 shows the changes in the activity of main algae and predators under different pH values.

[0036] Table 1 Vitality states of host algae and predators at different pH values

[0037]

[0038] It can be seen from Table 1 that when the resuscitation fluid of this ratio is at a ...

Embodiment 2

[0051] A kind of algae resuscitating liquid for shellfish feeding on microalgae, characterized in that it comprises the following components by weight:

[0052] 1000 parts of potassium nitrate;

[0053] 500 parts of potassium dihydrogen phosphate;

[0054] 300 parts of disodium edetate;

[0055] 100 parts of ferric chloride;

[0056] 200 parts of sodium bicarbonate;

[0057] Vitamin B 1 10 copies;

[0058] Vitamin B 12 20 copies;

[0059] 2 parts of trace elements, wherein the trace elements are configured by boric acid, manganese chloride, and zinc chloride in a ratio of 1:1:1;

[0060] 1500 parts of distilled water.

[0061] The resuscitation solution was formed by mixing the above raw materials, and then HCL was added to the resuscitation solution to adjust the pH value of the resuscitation solution. In the case of different pH values, the changes in the activity of main algae and predators are shown in Table 3.

[0062] Table 3 Vitality states of host algae and pr...

Embodiment 3

[0077] A kind of algae resuscitating liquid for shellfish feeding on microalgae, characterized in that it comprises the following components by weight:

[0078] 750 parts of potassium nitrate;

[0079] 750 parts of potassium dihydrogen phosphate;

[0080] 250 parts of disodium edetate;

[0081] 150 parts of ferric chloride;

[0082] 150 parts of sodium bicarbonate;

[0083] Vitamin B 1 15 copies;

[0084] Vitamin B 12 15 copies;

[0085] 3 parts of trace elements, among which trace elements are configured by boric acid, manganese chloride, zinc chloride, cobalt chloride, and ammonium molybdate in a ratio of 2:1:1:1:1;

[0086] 1800 parts of distilled water.

[0087] The resuscitation fluid was formed by mixing the above raw materials, and then HCL was added to the algae fluid to adjust the pH value of the algae fluid. Table 5 shows the changes in the vigor of the main algae and predators at different pH values.

[0088] Table 5 Vitality states of host algae and predat...

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Abstract

The invention belongs to the technical field of algological biology. In view of defects in the prior art, the invention provides an algae filament resuscitation fluid for shellfish feeding microalgae,which is characterized by comprising the following components in parts by weight: 500-1000 parts of potassium nitrate, 500-1000 parts of potassium dihydrogen phosphate, 200-300 parts of ethylene diamine tetraacetic acid, 100-200 parts of ferric trichloride, 100-200 parts of sodium bicarbonate, 10-20 parts of vitamin B1, 10-20 parts of vitamin B12, 2-5 parts of trace elements and 1500-2000 parts of distilled water. The algae filament resuscitation fluid for the shellfish feeding microalgae provided by the invention is safe and reliable, has no adverse effect on the growth of the shellfish feeding microalgae, avoids the waste of a culture solution caused by algae pollution, and effectively reduces the production cost of the shellfish seedling industry.

Description

technical field [0001] The invention belongs to the technical field of algae biology, and in particular relates to an algae species resuscitation liquid for shellfish feeding on microalgae. Background technique [0002] Shellfish need to take in a lot of nutrients in the process of growth and development, and mainly rely on the intake of nutrients from microalgae to maintain growth. hot issues of concern. Although the traditional algae-feeding medium can guarantee the basic nutrients for shellfish growth, it cannot ensure the growth and reproduction efficiency of algae, and is easily affected by miscellaneous algae and diseases, causing algae pollution. [0003] CN102093957A discloses a high-fat chlorella culture solution for rapid growth promotion. It contains bamboo acetic acid 1-8%, main nutrient 4-15%, micronutrient 0.2-2.5%, soil extract 0-1.5%, water 78-94%, and described main nutrient is sodium nitrate, One or more mixtures of dipotassium hydrogen phosphate, potass...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 刘建玲常丽荣闫欣杨晓斌卢龙飞刘力源李长青
Owner 威海长青海洋科技股份有限公司
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