Soluble two-photon fluorescent probe for detecting benzoyl peroxide in flour and living bodies and preparation method and application thereof

A two-photon fluorescence and benzoyl peroxide technology is applied in the field of chemical analysis and detection, and achieves the effects of broad application prospects, simple synthesis and low synthesis cost.

Inactive Publication Date: 2019-02-12
HUNAN UNIV OF TECH
View PDF6 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This new compound described by this inventor allows for easy creation of an easily accessible molecule that shows both bone resoφtion activity (BPA) and oxidative stress response pathways at once - it also works well on other substances such as glucose or pH levels. It'll be useful tools during medical researches like drug development because they are able to measure how much these chemical agents affect healthy individuals while being less expensive than current methods.

Problems solved by technology

This patented technical problem addressed in this patents relates to improving the accuracy and specificity of fluorescence techniques that use certain compounds called bphenol or naphthoquinones to measure cellular damage caused by radiation exposure during medical procedures such as X ray photography scans.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Soluble two-photon fluorescent probe for detecting benzoyl peroxide in flour and living bodies and preparation method and application thereof
  • Soluble two-photon fluorescent probe for detecting benzoyl peroxide in flour and living bodies and preparation method and application thereof
  • Soluble two-photon fluorescent probe for detecting benzoyl peroxide in flour and living bodies and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] This embodiment provides a method for preparing a soluble two-photon fluorescent probe for flour and in vivo detection of benzoyl peroxide, comprising the following steps:

[0038] S1. Synthesis of crude product: 0.193g (1.00mmol) 4-(diethylamino)-salicylaldehyde, 0.186g p-benzyl bromide phenyl borate (1.00mmol), 0.294g (3mmol) K 2 CO 3 and 40mL CH 3 CN was added to a 100-ml round-bottomed flask, equipped with a circulating condensing device, the mixture was stirred at 65 ° C, and the reaction was followed by thin-layer chromatography until 4-(diethylamino)-salicylaldehyde was completely reacted; the final mixture Pour into an appropriate amount of ice water, and suction filter to obtain a yellow solid. The solid is washed 3 times with cold water, and the solid is dried in a constant temperature drying oven to obtain a solid crude product that is directly used in the next reaction. The structure of the crude product is as shown in formula (II). Shown, yield 90%.

[0...

Embodiment 2

[0044] The present embodiment is a probe molecular detection test, and the results are as follows: image 3 shown. in:

[0045] (a) UV-Vis absorption spectra normalized for CM-1 and CM-1+BPO.

[0046] (b) is the fluorescence spectrogram of the response of the probe molecule (1 μM) to BPO, BPO (0-35 μM), the titration experiment between the BPO fluorescent probe and BPO. Use a fluorescence spectrometer to test the fluorescence spectrum under different concentrations of BPO. The excitation wavelength of the fluorescence spectrum is 380nm, the emission wavelength is 500nm, and the detection wavelength is 400-650nm. With the increase of the concentration of BPO, the fluorescence intensity at the wavelength of 500nm gradually increases. , indicating that the fluorescent probe prepared by the present invention can respond to BPO.

[0047] (c) is the calibration curve.

[0048] (d) is a linear response.

[0049] (e) and (f) are the visual detection of BPO by fluorescent probes. ...

Embodiment 3

[0051] This embodiment is a selectivity test for fluorescent probe detection of BPO, and the test results are as attached Figure 4 shown.

[0052] Under the same test conditions, the fluorescence spectrum after adding 35 micromoles of BPO or 100 micromoles of other analytes to 1 micromoles of probe molecules, wherein: the excitation wavelength of the fluorescence spectrum is 380nm, the emission wavelength is 500nm, and the detection wavelength is 400-650nm. The results are attached Figure 4 (a) shown.

[0053] attached by Figure 4 (a) It can be seen that numbers 1 to 16 represent blanks (probe molecules) respectively, and S 2 o 3 2- , S 2 o 4 2- , S 2 o 5 2- , SO 3- , GSH, Cys, Hcy, Na 2 S, Ca 2+ , Mg 2+ , Na + , K + , Cu 2+ , Zn 2+ , and Fe 3+ . The fluorescence intensity is only significantly enhanced by BPO, and other bioactive small molecules and other analytes do not interfere with the detection results, indicating that the fluorescent probe prepar...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the field of chemical analysis and detection, and particularly discloses a water-soluble two-photon fluorescent probe capable of being directly used for detecting benzoyl peroxide in flour, cancer cells and tissues and a preparation method and application thereof. The fluorescent probe adopts coumarin derivatives with a two-photon property as a signal group and the p-phenylborate benzyl ether as a response recognition group of BPO. The structure of the fluorescent probe is represented by a formula (I). The fluorescent probe has good water solubility, high selectivity,superior anti-interference ability and high sensitivity, can be directly used for rapid detection of BPO in the flour, cancer cells and tissues, and has good application prospects.

Description

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Owner HUNAN UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap