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Application of osrl3.3 protein in regulating plant root development

A protein and root number technology, applied in the fields of application, plant peptides, plant gene improvement, etc., can solve problems such as affecting yield, affecting the full use of cultivated land resources, and failing to effectively use them.

Active Publication Date: 2021-08-03
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Research shows that most of the water consumption of irrigated rice is not required for growth itself, but because it is not used efficiently
On the other hand, nearly half of the world's rice planting area is in a state of water shortage, which seriously affects the increase in its yield. In many areas of my country, due to the relative lack of irrigation water, it is difficult to develop rice production, which affects arable land resources to a certain extent. full use of

Method used

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  • Application of osrl3.3 protein in regulating plant root development
  • Application of osrl3.3 protein in regulating plant root development
  • Application of osrl3.3 protein in regulating plant root development

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1, the acquisition of rice OsRL3.3 and its sequence analysis

[0049] 1. Obtaining of rice OsRL3.3

[0050]Using 795 seedling stage cultivated rice germplasms (including 506 indica and 289 japonica) as the association analysis population, the genotype and root length phenotype of the association analysis population were analyzed and identified. Specific steps are as follows:

[0051] 1. Phenotypic identification of the root length of the association analysis population by hydroponics

[0052] 1) First, after washing the seeds with sterile water, place them in a tissue culture room at 32°C for 64 hours to accelerate germination.

[0053] 2) For each variety, 5 seeds with normal germination and consistent growth potential were selected and transplanted to foam boards (30cm×42cm) with gauze as the bottom. Each foam board can plant 22 varieties (110 plants) and 20 rice plants as protection rows, and every two foam boards can be placed in the same blue plastic box...

Embodiment 2

[0060] Example 2, Obtaining of Rice OsRL3.3 Mutant and Functional Analysis of OsRL3.3

[0061] 1. Obtaining of rice OsRL3.3 mutant

[0062] Mutant Ti-OsRL3.3 was purchased from Korea Mutant Bank (URL: http: / / cbi.khu.ac.kr / RISD_DB.html), catalog number PFG_1D-03332.L.

[0063] 2. Functional analysis of rice OsRL3.3

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Abstract

The invention discloses the application of OsRL3.3 protein in regulating plant root development. The OsRL3.3 protein is the protein of a) or b) or c) or d) as follows: a) the amino acid sequence is the protein shown in sequence 2; b) it is connected at the N-terminal and / or C-terminal of the protein shown in sequence 2 The fusion protein obtained by labeling; c) the protein with the same function obtained by substituting and / or deleting and / or adding the amino acid sequence shown in sequence 2 through one or several amino acid residues; d) the same function as shown in sequence 2 Proteins with 75% or more homology in amino acid sequence and the same function. It is proved by experiments that the mutation of OsRL3.3 gene can affect the normal development of rice root system, the gene has the function of regulating the development of rice root and primary root, and can be used to improve the traits of rice root system.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to the application of OsRL3.3 protein in regulating plant root development, in particular to the application of OsRL3.3 protein in regulating rice root development. Background technique [0002] Rice is one of the three major food crops in the world. Irrigated rice is currently the main rice production method, and its water consumption accounts for about 54% of the national water consumption and more than 65% of the national agricultural water consumption. Studies have shown that most of the water consumption of irrigated rice is not required for growth itself, but due to ineffective use. On the other hand, nearly half of the rice planting area in the world is in a state of water shortage, which seriously affects the increase of its yield. In many areas of my country, due to the relative lack of irrigation water, it is difficult to develop rice production, which affects arabl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29
CPCC07K14/415
Inventor 李自超赵炎蒋聪慧邵雨洁赵为朋张洪亮李金杰
Owner CHINA AGRI UNIV
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