Application of OsRL3.3 protein to regulation and control of plant root development
A technology of plant roots and plants, applied in the fields of application, plant peptides, plant gene improvement, etc., can solve the problems of difficulty in developing rice production, affecting the full utilization of cultivated land resources, and affecting yield.
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Embodiment 1
[0048] Example 1, the acquisition of rice OsRL3.3 and its sequence analysis
[0049] 1. Obtaining of rice OsRL3.3
[0050]Using 795 seedling stage cultivated rice germplasms (including 506 indica and 289 japonica) as the association analysis population, the genotype and root length phenotype of the association analysis population were analyzed and identified. Specific steps are as follows:
[0051] 1. Phenotypic identification of the root length of the association analysis population by hydroponics
[0052] 1) First, after washing the seeds with sterile water, place them in a tissue culture room at 32°C for 64 hours to accelerate germination.
[0053] 2) For each variety, 5 seeds with normal germination and consistent growth potential were selected and transplanted to foam boards (30cm×42cm) with gauze as the bottom. Each foam board can plant 22 varieties (110 plants) and 20 rice plants as protection rows, and every two foam boards can be placed in the same blue plastic box...
Embodiment 2
[0060] Example 2, Obtaining of Rice OsRL3.3 Mutant and Functional Analysis of OsRL3.3
[0061] 1. Obtaining of rice OsRL3.3 mutant
[0062] Mutant Ti-OsRL3.3 was purchased from Korea Mutant Bank (URL: http: / / cbi.khu.ac.kr / RISD_DB.html), catalog number PFG_1D-03332.L.
[0063] 2. Functional analysis of rice OsRL3.3
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