Peripheral blood mononuclear cell separation tube and preparation method thereof

A nuclear cell and separation tube technology, applied in the field of separation, purification of cell components, and blood sampling, can solve the problems of long time-consuming, different, and many operating procedures, so as to simplify the operation process, reduce inconsistency, and reduce the cost of experimental equipment Effect

Inactive Publication Date: 2019-01-25
珠海朗泰生物科技有限公司
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Problems solved by technology

[0013] From the above steps, it can be seen that the Ficoll-hypaque density gradient centrifugation method has the following disadvantages: 1) There are many operating procedures and time-consuming; 2) Carefully put the Ficoll-hypaque solution under the diluted blood, and the second layer must be Avoid mixing, this step must have very skilled operation skills; 3) inconsistent centrifugation conditions will cause differences in the recovery rate and purity of PBMCs; 4) even if different operators use the same method to separate the purity and recovery rate of PBMCs. Will be different, poor repeatability

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  • Peripheral blood mononuclear cell separation tube and preparation method thereof
  • Peripheral blood mononuclear cell separation tube and preparation method thereof
  • Peripheral blood mononuclear cell separation tube and preparation method thereof

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preparation example Construction

[0057] Introduce specifically below, the preparation method of peripheral blood mononuclear cell of the present invention, comprises the following steps:

[0058] Preparatory work: Dip-coat a layer of polyether-modified siloxane on the inner wall of a φ16(mm)*125(mm) glass tube, and then dry it at 120-150°C for 2 hours to obtain a bionic film on the inner wall of the tube.

[0059]S1 Base resin for synthesizing the separation gel: weigh α-olefin, dialkyl maleate and tert-butyl peroxybenzoate by weight ratio; mix the α-olefin and dialkyl maleate evenly, add After heating the four-necked flask to 140-160°C, add tert-butyl peroxybenzoate in a dropwise manner for 3-4 hours; The temperature rises to 190°C, and then the volatile substances and unreacted monomers are distilled off by vacuum distillation to obtain the matrix resin;

[0060] S2 Synthetic separation gel: Weigh and mix the matrix resin, nano silicon dioxide and polyoxyethylene polyoxypropylene ether according to the wei...

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Abstract

The invention relates to a peripheral blood mononuclear cell separation tube and a preparation method thereof, comprising a tube body and a tube plug, wherein the inner part of the tube body comprisesFicoll-hypaque stratified liquid layer, separating the gel layer and the anticoagulant layer; in the tube body, an upper space of the separating glue layer is used for accommodating sample blood. Compared with the prior art, the use of the peripheral blood mononuclear cell separation tube of the present invention eliminates the need for transferring blood from the vacuum collection tube into theconical tube, and eliminates the need for transferring Ficoll-hypaque stratification fluid is carefully injected into the lower part of the blood, and the PBMCs layer is sucked from the solid separating gel interface and yellow plasma without being carefully sucked from the liquid interface. Thus, the experimental apparatus is reduced, the operation process is simplified, the operation difficultyis reduced, and the inconsistency among different operators is reduced.

Description

technical field [0001] The invention belongs to the field of blood sampling, separation and purification of cell components, and in particular relates to a peripheral blood mononuclear cell separation tube and a preparation method thereof. Background technique [0002] Peripheral Blood Mononuclear Cells (PBMCs) refer to cells with a single nucleus in peripheral blood, including a variety of lymphocytes and monocytes, and are an important part of the immune system. Specifically, peripheral blood mononuclear cells include about 15% of B lymphocytes, about 70% of T lymphocytes, about 10% of natural killer cells, and about 5% of monocytes. [0003] Currently, the most classic method to obtain PBMCs from whole blood is Ficoll-hypaque density gradient centrifugation. Ficoll-hypaque layering solution is a product of GE Company, which is a solution with a certain viscosity and specific gravity, which is close to isotonicity. Specifically, the Ficoll-hypaque layering solution is a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/24C12N5/078
CPCC12N5/0634C12N2509/00
Inventor 陈琼娣刘强付银鑫廖南山马海波林锋李洁
Owner 珠海朗泰生物科技有限公司
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