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Natural enemy mew audio induction anxiety animal model construction method and application

A technology of animal models and construction methods, applied in animal husbandry, etc., can solve problems such as difficulty in achieving control levels, difficulties in rodent experiments, and easy influence on other experiments, achieving high accuracy, significant effects, and solving the effects of cumbersome production

Inactive Publication Date: 2019-01-25
WUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in practice, the microbial control level of experimental animals with natural enemies is difficult to meet the requirements of the barrier system for rodents, which brings difficulties for rodents to test in the barrier system; and improper operation of natural enemy odors can easily affect other tests.

Method used

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  • Natural enemy mew audio induction anxiety animal model construction method and application
  • Natural enemy mew audio induction anxiety animal model construction method and application
  • Natural enemy mew audio induction anxiety animal model construction method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Induction of anxiety animal models

[0023] In the quiet animal behavior testing room, the natural enemy cat meowing audio was played through the audio player within the rat's hearing range. The main frequency of the audio is 2.541kHz, and the power spectrum of the audio is as follows figure 1 As shown, the spectrogram is as figure 2 shown. The audio player is within 2 meters from the rat, and the audio intensity is set to 60dB for 5 minutes. In order to avoid being disturbed by the sound of cats meowing from natural enemies before the test, the animal breeding room is separated from the testing laboratory, and each animal will not enter the testing laboratory until the test.

[0024] (2) Open field test

[0025] While playing the natural enemy meowing audio, the rat was placed in the lower right corner of the independent space of the square open open field box, with its head facing the corner. The schematic diagram of the open field box is shown in image 3 ...

Embodiment 2

[0029] (1) Induction of anxiety animal models

[0030] In a quiet room, the natural enemy meowing audio was played through the audio player within the hearing range of the rat. The audio source was within 2 meters from the rat, and the audio intensity was set to 80dB for 7 minutes. In order to avoid being disturbed by the sound of cats meowing from natural enemies before the test, the animal breeding room is separated from the testing laboratory, and each animal will not enter the testing laboratory until the test.

[0031] (2) Open field test

[0032] While playing the natural enemy meowing audio, place the rat in the lower right corner of the independent space of the open field box, with its head facing the corner, observe and analyze its autonomous activities in the open field box for 5 minutes through the animal video monitoring system, record and analyze Indicators include: average speed, the proportion of distance traveled in the central area, and the proportion of dwel...

Embodiment 3

[0036] (1) Induction of anxiety animal models

[0037] In a quiet room, the natural enemy meowing audio was played through the audio player within the hearing range of the rat. The audio source was within 2 meters of the rat, and the audio intensity was set to 60dB for 10 minutes. In order to avoid being disturbed by the cat meowing audio of natural enemies before the test, the distance between the animal breeding room and the test laboratory is relatively long. After the cat-calling audio stimulation by the natural enemy, the rats were put back into the animal room and continued to be raised for 7 days.

[0038] (2) Elevated plus maze detection

[0039] On the 7th day after the stimulation treatment, the rats were placed in the central platform area of ​​the elevated plus maze with their heads facing the closed arms, and their average speed, distance percentage of the open arm area and open arm area were observed and analyzed by the animal video monitoring system within 5 mi...

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Abstract

The invention discloses a natural enemy mew audio induction anxiety animal model construction method. Natural enemy mew audio frequencies are broadcast within the hearing domain of an experiment mousethrough an audio player, and an anxiety animal model is induced; while the natural enemy mew audio frequencies are played or within follow-up seven days, animal behavior testing is performed on the experiment mouse to evaluate the anxiety behavior. The invention further discloses application of the anxiety animal model obtained through the construction method for screening anti-anxiety drugs. Thenatural enemy mew audio induction anxiety animal model is remarkable in effect, convenient to use and safe, the obtained experiment result is high in accuracy, and the method can be used for screening the anxiety drugs and evaluating and treating the anxiety disorder.

Description

technical field [0001] The invention belongs to a construction method and application of an animal model, in particular to a construction method and application of an anxiety animal model induced by a natural enemy meowing audio. Background technique [0002] Anxiety disorders are a type of mental illness characterized by episodic or persistent emotional anxiety, accompanied by symptoms such as autonomic disturbance, muscle tension, and restlessness in movement. In recent years, with the increasingly fierce social competition and the increasing pressure of life, the incidence of anxiety disorders has increased year by year, and has become one of the main mental diseases that affect people's quality of life, bringing a heavy burden to patients' families and society. The etiology of anxiety disorders is still unclear, and anxiolytic drugs are limited in clinical practice due to many adverse reactions. Therefore, anxiety disorders and new anti-anxiety drugs have become the foc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 陈超杰钟志凤
Owner WUZHOU UNIV
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