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Application of lectin probe combination to urine protein glycotype-based gender identification of Qinling rhinopithecus roxellana

A technology of lectin and golden monkey, which is applied in the direction of material inspection products, instruments, analytical materials, etc., can solve the problem of not getting enough complete and clear sugar chain distribution information, and achieve the effect of low cost

Active Publication Date: 2019-01-18
NORTHWEST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Studies have found that the sugar chains in human serum, saliva, and urine are different between men and women, but there is still not enough complete and clear sugar chain distribution information

Method used

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  • Application of lectin probe combination to urine protein glycotype-based gender identification of Qinling rhinopithecus roxellana
  • Application of lectin probe combination to urine protein glycotype-based gender identification of Qinling rhinopithecus roxellana
  • Application of lectin probe combination to urine protein glycotype-based gender identification of Qinling rhinopithecus roxellana

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Experimental program
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Embodiment Construction

[0021] 1. Experimental part

[0022] 1.1 Reagents and materials

[0023] Glycine, disodium hydrogen phosphate, sodium dihydrogen phosphate, Tween-20 and protease inhibitors were all purchased from Sigma-Aldrich, USA. Bovine serum albumin (BSA) was purchased from Merck, Germany. Cy3 fluorescence was purchased from Amerhsma, USA. Other chemical reagents were of analytical grade without further purification before use. All experimental water was ultrapure water treated by Milli-Q50 pure water system (Millipore, USA). Sephadex G-25 column desalting was purchased from GE Healthcare, USA. Chip hybridization kit was purchased from Bio-Rad, USA. Other common glassware are all made in China. Thirty-seven lectins (see Table 1 for specific names) were purchased from Sigma-Aldrich and Vector, Germany, respectively.

[0024] Table 1: Names of lectins and their specific recognition sugar chains

[0025]

[0026]

[0027]

[0028] 1.2 Experimental Instruments

[0029] Elect...

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Abstract

The invention provides an application of a lectin probe combination to the urine protein glycotype-based gender identification of Qinling rhinopithecus roxellana. The lectin probe combination is a combination of PNA, VVA, BPL, AAL and PTL-I; and if a sample detection result is that PNA and VVA expression is down-regulated and BPL, AAL and PTL-I expression is up-regulated, it is showed that corresponding subjects are the female Qinling rhinopithecus roxellana, otherwise, the corresponding subjects are the male Qinling rhinopithecus roxellana. The lectin probe combination is used for identifyingthe genders of the Qinling rhinopithecus roxellana; and the lectin probe combination is rapid, simple and convenient, accurate and relatively low in implementation cost.

Description

technical field [0001] The invention relates to a method for distinguishing the sex of Qinling Sichuan golden monkey (adult). Background technique [0002] In order to improve the productivity of domestic animals, sex control of mammals has become an important content in animal husbandry research. People often hope to produce animals of the expected sex, thereby promoting the advancement of animal genetics and breeding technology, strengthening the prevention and treatment of associated diseases, and better utilization of resources. thereby serving humanity itself. In the study of wild animals, the sex ratio is one of the basic contents of the study of animal population ecology, and the individual ratio of male and female animals in an animal population plays an important role in revealing the population structure. However, in the actual wildlife protection research, it is difficult for us to observe many animals at close range. Scholars have explored a variety of methods t...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N21/64
CPCG01N21/6428G01N21/6486G01N33/6827
Inventor 郭松涛张思萌雷颖虎何刚张河陈瑞于汉杰张宸李铮李保国
Owner NORTHWEST UNIV
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