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Chrysopa pallens insulin-like peptide-4 gene cDNA and application thereof

An insulin-like, large lacewing technology, applied in the field of insect physiology and molecular biology, can solve problems that do not involve Neuropteran insects, and do not involve the application of insulin-like peptides, and achieve the effect of prolonging the oviposition period and increasing the amount of oviposition

Inactive Publication Date: 2019-01-15
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the research work on insect insulin-like peptides is still in its infancy, and the research objects are mainly limited to a few species of Diptera, Hymenoptera, Coleoptera, Lepidoptera, and Hemiptera, and no Neuroptera insects have been involved.
Moreover, related reports only explored the physiological functions of insulin-like peptides, and did not involve the application of insulin-like peptides in insect feeding

Method used

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  • Chrysopa pallens insulin-like peptide-4 gene cDNA and application thereof
  • Chrysopa pallens insulin-like peptide-4 gene cDNA and application thereof

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Embodiment Construction

[0014] The present invention will be further clarified through the detailed description of specific embodiments below, but it is not intended to limit the present invention, but only for illustration.

[0015] The present invention clones the insulin-like peptide-4 gene cDNA from the lacewing, which has the sequence shown in SEQ ID NO.1:

[0016] (1) Information of SEQ ID NO.1

[0017] (a) Sequential features

[0018] Sequence length: 420 base pairs

[0019] Type: nucleic acid

[0020] Chain type: double chain

[0021] Topology: Linear

[0022] (b) Molecular type: cDNA

[0023] (c) Assumption: No

[0024] (d) Antisense: No

[0025] (e) Original source: Chrysopa pallens

[0026] (f) Sequence description: SEQ ID NO.1

[0027] (2) Information of SEQ ID NO.2

[0028] (a) Sequential features

[0029] Sequence length: 140 amino acids

[0030] Type: amino acid

[0031] Chain type: single chain

[0032] Topology: Linear

[0033] (b) Molecule type: protein

[0034] (c) ...

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Abstract

Insect insulin-like peptides (ILPs) are widely involved in the regulation of insect growth, development, reproduction and other physiological processes. Chrysopa pallens is an important predatory natural enemy insect. The invention discloses an insulin-like peptide of Chrysopa pallens insulin-like peptide-4 gene cDNA. The Chrysopa pallens insulin-like peptide-4 having bioactivity is obtained by synthesis and assembling in vitro. 4. For female adults of Chrysopa pallens fed on insulin-like peptide-4, the egg production is increased by 22.6%. The Chrysopa pallens insulin-like peptide-4 can be used to regulate the physiological processes such as oviposition, diapause and senescence of Chrysopa pallens and other insects, and has a good application prospect in the large-scale production of insects.

Description

technical field [0001] The invention belongs to the field of insect physiology and molecular biology, and in particular relates to a cDNA of the lacewing insulin-like peptide-4 gene and application thereof. Background technique [0002] Lacewings are important predatory natural enemies of agricultural, forestry, fruit and vegetable pests in my country, belonging to Neuroptera. The large lacewing has a large predation capacity, a wide distribution, strong adaptability, and both adults and larvae can prey, so it has great application potential. Natural enemy insects, including lacewings, occupy an important strategic position in the field of biological control of agricultural pests. [0003] Vertebrate insulin (Insulin) plays an important role in the process of glucose metabolism and has been widely used in the treatment of diabetes. Insects can also synthesize protein hormones similar to vertebrate insulin, called insulin-like peptides (Insulin-like Peptides, ILPs). Studie...

Claims

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Application Information

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IPC IPC(8): C12N15/12C07K14/435
CPCA23K20/147A23K50/90C07K14/43563
Inventor 毛建军张国财张婷婷
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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