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A strain of Bacillus mohaevei for promoting the growth of Thoreau grass and application thereof

A technology of Bacillus weiba and Bacillus, applied in the field of biological pesticides, can solve problems such as single bacteria

Active Publication Date: 2019-01-11
QINGHAI UNIVERSITY
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  • Application Information

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Problems solved by technology

The most important thing is that the genus of the existing biocontrol strains is too single, mainly concentrated in Bacillus subtilis and Bacillus amyloliquefaciens. Whether there are other biocontrol strains that are resistant to extreme environments and can effectively promote the growth of T. main content

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  • A strain of Bacillus mohaevei for promoting the growth of Thoreau grass and application thereof
  • A strain of Bacillus mohaevei for promoting the growth of Thoreau grass and application thereof
  • A strain of Bacillus mohaevei for promoting the growth of Thoreau grass and application thereof

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Embodiment 1

[0029] 1.1 Isolation of strains

[0030] The applicant isolated and screened a rhizosphere soil of Androsace umbellata in the saline-alkali site of Hoh Xil, Haixi Prefecture, Qinghai Province (altitude: 5500m, annual average temperature is -10.0°C to 4.1°C, and annual average precipitation is 173 to 495mm). strain and named it KKDD. The separation and purification of the bacterial strain KKDD of the present invention adopt methods such as dilution coating plate method, plate streaking and the like.

[0031] 1.2 Identification of strains

[0032] 1.2.1 Morphological identification of strains

[0033] By observing the colony shape, color, edge, transparency, etc., the strain KKDD was streaked on LB (Luria-Bertan) medium. Such as figure 1 As shown, the main morphological characteristics of strain KKDD are: the optimum growth temperature on LB medium is 25°C, and after 1-2 days of culture, colonies with a diameter of 2-3 cm are formed, the colonies are milky white, opaque, and...

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Abstract

The invention discloses a strain of Bacillus mohaevei for promoting the growth of Thoreau grass and the application thereof. The strain KKDD belongs to Bacillus mojavensis (Bacillus mojavensis), is deposited at the China General Microbiological Culture Collection Center on 11 April 2018, strain collection No. CGMCC NO. 15579(address: No. 3 Yuan, No. 1 Beichen West Road, Chaoyang District, Beijing)Bacillus rhizospheres KKDD isolated from saline-alkali vegetation in Hoh Xil, Qinghai province, has that characteristics of nitrogen fixation, antagonism to pathogen, IAA production, germination promotion and saline-alkali tolerance, and has certain research and application potential in grassland vegetation restoration and ecological agriculture and animal husbandry in plateau. The KKDD strain can not only obviously improve the growth of Thoreau grass, but also degrade straw lignocellulose and antagonize the fungal diseases of Thoreau grass.

Description

technical field [0001] The invention belongs to the technical field of biopesticides, and in particular relates to a bacillus mohaiwei strain and its application in preventing and treating fungal diseases of Thorax weed and promoting the growth of Thorax weed. Background technique [0002] Solograss is a perennial herb belonging to the family of Gramineae, Triticum genus. In my country, Solograss is concentrated in Qinghai, Tibet and other places. Cool at high altitude. Thoreau grass has strong soil-fixing ability, cold and drought resistance, wind and sand resistance, and salt-alkali tolerance. It is suitable for the alpine grassland habitat with harsh climate conditions. It is one of the main components of the alpine grassland plant community and can be used for ecological restoration. As the tholograss is rich in nutrition and easy for animals to digest, it can be used as feed, thereby alleviating the phenomenon of insufficient feed after the pastures on the Qinghai-Tibet...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P21/00A01P3/00C05F11/08A01C1/00A01C21/00A01G7/06C12R1/07
CPCA01C1/00A01C21/005A01G7/06A01N63/00C05F11/08C12N1/20C12N1/205C12R2001/07
Inventor 谢永丽吴晓晖陈兰柴树芳
Owner QINGHAI UNIVERSITY
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