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Rapid dewaxing device for FFPE sample and using method of FFPE rapid dewaxing device

A sample and dewaxing technology, applied in the preparation of test samples, etc., can solve the problems of difficult to automate the dewaxing process, complex dewaxing process, large workload, etc., achieve convenient and rapid separation processing, avoid aerosol pollution, Automate the effect

Inactive Publication Date: 2019-01-04
SHANGHAI MAG GENE NANOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the above-mentioned defects of the prior art, the technical problem to be solved by the present invention is that the current FFPE sample dewaxing process is complicated, the workload is large and it is difficult to realize the automation of the dewaxing process

Method used

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  • Rapid dewaxing device for FFPE sample and using method of FFPE rapid dewaxing device
  • Rapid dewaxing device for FFPE sample and using method of FFPE rapid dewaxing device
  • Rapid dewaxing device for FFPE sample and using method of FFPE rapid dewaxing device

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Experimental program
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Effect test

Embodiment 1

[0034] Manually operated FFPE dewaxing device and process:

[0035] There is a sealing device above the sample incubation chamber 101, and the sample incubation chamber 101 is prepackaged with 200 μL tissue lysate, 20 μL proteinase K and 50 μL oil phase reagents, such as mineral oil, paraffin oil, vegetable oil and silicone oil.

[0036] The FFPE tissue sample is put into the sample incubation chamber 101 after opening the sealing device above the sample incubation chamber 101, and the sealed state is maintained again by closing the lid.

[0037] The volume of the sample storage chamber 106 is 200 μL, and the sample storage chamber 106 can be removed from the whole device and stored with a lid on.

[0038] Tissue lysate, proteinase K and oil phase reagents are all commercially available.

[0039] According to the aforementioned dewaxing process (see schematic Figure 1-3 ), after the sample storage chamber 106 is full, close the valve 105, remove the sample storage chamber 1...

Embodiment 2

[0041] FFPE dewaxing device and process connected to the subsequent automatic nucleic acid extraction device:

Embodiment 3

[0042] The schematic diagram of the device used in embodiment 3 is shown in the schematic Figure 4 .

[0043] The FFPE tissue sample is put into the device through the opening and closing device (not shown here) above the sample incubation chamber 101 .

[0044] The sample incubation chamber 101 includes 500 μL tissue lysate, 10 μL proteinase K and 100 μL oil phase reagent.

[0045] One end of the sample channel 109 is connected to the valve 105, and the other end can be connected to the sample chamber of the subsequent nucleic acid extraction device.

[0046] The driving pump 110 is a schematic liquid driving device, which can pump the liquid in the sample incubation chamber 101 to the subsequent sample chamber through the sample channel 109 .

[0047] The valve 105 contains a filter membrane (not shown here), which can filter unlysed tissue fragments and oil phase liquid in the water phase layer 108 .

[0048] Tissue lysate, proteinase K and oil phase reagents are all co...

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Abstract

The invention discloses a rapid dewaxing device for an FFPE sample, and relates to the field of dewaxing of FFPE samples. The rapid dewaxing device for the FFPE sample comprises a sample incubation cavity and a heating module, wherein the sample incubation cavity is connected with the heating module; the heating module is set to heat the sample incubation cavity to a specific temperature; and a closable opening is formed in the upper portion or a side surface of the sample incubation cavity. In combination an FFPE dewaxing splitting method which consumes short time and incubates at the same temperature only, the automatic dewaxing device for the FFPE sample can be integrated in an automatic device, and the FFPE sample can be subjected to closed dewaxing operation by manual operation or anautomatic instrument. The device is simple to operate, and is easily integrated into an automatic instrument, and a convenient and rapid integrated solution is provided for simplifying operation complexity of treatment of the FFPE sample at present.

Description

technical field [0001] The invention relates to the field of FFPE dewaxing, in particular to an FFPE sample rapid dewaxing device and a use method thereof. Background technique [0002] Formalin-Fixed and Paraffin-Embedded (FFPE) is a tissue preservation technique. In order to maintain the nuclear protein structure of this technique, the tissue is first fixed with formalin and then embedded in solid paraffin for histological diagnosis or research after sectioning. Due to its advantages of fully maintaining the original tissue morphology of the sample and long-term storage, it is currently widely used in clinical pathological diagnosis, pathological mechanism research, and cancer drug guidance. [0003] However, if you want to conduct molecular biology research or application on FFPE tissue, you first need to extract nucleic acid. However, the fixation of formalin to the sample will lead to cross-linking of nucleic acid and protein, which will affect the extraction of nucle...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/36
CPCG01N1/36
Inventor 方晓霞徐宏古宏晨
Owner SHANGHAI MAG GENE NANOTECH CO LTD
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