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Rapid propagation method of cymbidium ensifolium seeds by aseptic sowing

A technology for aseptic seeding and seeding, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of limited provenance, degraded species, easy to carry viruses, etc., and achieve the effect of simplifying the production process and simple method

Inactive Publication Date: 2019-01-04
翁源县天下泽雨农业科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is to overcome the existing defects and provide a method for aseptic sowing and rapid propagation of Jianlan seeds, so as to solve the problem in the above-mentioned background technology that most domestic orchid planting enterprises still use the traditional Branch reproduction is the main method, and there are problems such as limited provenance, low reproductive efficiency, species degradation and easy virus carrying, which cannot meet the needs of large-scale industrial production

Method used

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  • Rapid propagation method of cymbidium ensifolium seeds by aseptic sowing

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Effect test

Embodiment 1

[0029] A kind of aseptic sowing rapid propagation method of Jianlan seeds, comprising the following steps:

[0030] Step 1. Seed material treatment and disinfection process; take the mature and uncracked capsules of Jianlan 9, wash them with running water, then wipe the Jianlan capsules with cotton pads dipped in 75% alcohol, then soak and shake them in 10 times bleach water for 15 minutes, and then use sterile Rinse with water 3-4 times, dry the water, cut a small opening of the capsule with a sterile scalpel, and sprinkle the seeds in the capsule evenly on the medium: American Huabao No. 1 1--2g / L+Potato 10-20g / L+banana 5-10g / L+activated carbon 1g / L.

[0031] Step 2. Seed germination and cultivation; cultivate in the dark for 2 months, then culture under weak light, and then cultivate in a lamp tube. After 6 months, the seeds will expand, and a protocorm will be formed first, and a protocorm will differentiate into a branched root. shape stem.

[0032] Step 3: Proliferati...

Embodiment 2

[0037]A kind of aseptic sowing rapid propagation method of Jianlan seeds, comprising the following steps:

[0038] Step 1. Seed material treatment and disinfection process; take the mature and uncracked capsules of Jianlan 9, wash them with running water, then wipe the Jianlan capsules with cotton pads dipped in 75% alcohol, then soak and shake them in 10 times bleach water for 25 minutes, and then use sterile Rinse with water 3-4 times, dry the water, cut a small opening of the capsule with a sterile scalpel, and sprinkle the seeds in the capsule evenly on the medium: American Huabao No. 1 2--3g / L+Potato 20-30g / L+banana 10-15g / L+activated carbon 2g / L.

[0039] Step 2. Seed germination and cultivation; cultivate in the dark for 2 months, then culture under weak light, and then cultivate in a lamp tube. After 6 months, the seeds will expand, and a protocorm will be formed first, and a protocorm will differentiate into a branched root. shape stem.

[0040] Step 3: Proliferati...

Embodiment 3

[0045] A kind of aseptic sowing rapid propagation method of Jianlan seeds, comprising the following steps:

[0046] Step 1. Seed material treatment and disinfection process; take the mature and uncracked capsules of Jianlan 9, wash them with running water, then wipe the Jianlan capsules with cotton pads dipped in 75% alcohol, then soak and shake them in 10 times bleach water for 35 minutes, and then use sterile Rinse with water 3-4 times, dry the water, cut a small opening of the capsule with a sterile scalpel, and sprinkle the seeds in the capsule evenly on the medium: American Huabao No. 1 3--4g / L+Potato 30-40g / L+banana 15-25g / L+activated carbon 3g / L.

[0047] Step 2. Seed germination and cultivation; cultivate in the dark for 2 months, then culture under weak light, and then cultivate in a lamp tube. After 6 months, the seeds will expand, and a protocorm will be formed first, and a protocorm will differentiate into a branched root. shape stem.

[0048] Step 3: Proliferat...

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Abstract

The invention discloses a rapid propagation method of cymbidium ensifoliumseeds by aseptic sowing. The rapid propagation method comprises the following steps: step 1, processes of treatment and disinfection to seeds: mature and unbroken capsules of cymbidium ensifolium 9 are taken and washed with running water, absorbent cottons soaked in alcohol with concentration of 75% are used for wiping the capsules of cymbidium ensifolium, and are then soaked in bleaching water with concentration of 10% and shaken for 15 minutes, aseptic water is used for washing the capsules of cymbidium ensifolium for3-4 times, the capsules of cymbidium ensifolium are aired under the sun for drying the water thereon, an aseptic scalpel is used for cutting a small opening on each capsule, and the seeds picked fromthe capsules are evenly spread in a culture medium which comprises 2-4 g / L of hyponex NO.1 imported from America, 10-50g / L of tomatoes, 5-25g / L of bananas and 1g / L of activated carbon. The success rate of disinfection to the capsules of cymbidium ensifolium can reach 100%, therefore the cymbidium ensifolium seeds are aseptically sowed and prompted to sprout for obtaining rhizomes, and the conditions that how to let the rhizomes grow and how to deferentially culture the rhizomes are discussed, so as to explore the rapid propagation technology of the cymbidium ensifolium Golden Lotus and the cymbidium ensifolium Summer Royal Titoni by aseptic sowing.

Description

technical field [0001] The invention belongs to the technical field of aseptic sowing of Jianlan seeds, and in particular relates to a method for aseptic sowing and rapid propagation of Jianlan seeds. Background technique [0002] Cymbidium ensifolium (Cymbidium ensifolium) is a terrestrial orchid plant in the genus Orchidaceae. It is one of the most important species of orchids. Its leaves are beautiful, the flowers are fragrant, and the flowering period is long. Called "Sijilan", Jianlan Jinhe and Jianlan Xiahuangmei are the more precious species among them, with high ornamental value and economic value. Born in sparse forests, thickets, valleys or grass, at an altitude of 600-1800 meters. Produced in Anhui, Zhejiang, Jiangxi, Fujian, Taiwan, Hunan, Guangdong, Hainan, Guangxi, southwestern Sichuan, Guizhou and Yunnan in China. Widely distributed in Southeast Asia and South Asia, north to Japan. The type specimen was collected from Guangdong. Orchids generally grow on t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 陈绪盛杜正丽毛明辉
Owner 翁源县天下泽雨农业科技有限公司
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