A kind of culture medium kit and method for in vitro rapid propagation of Pittonia chinensis

Abstract

The invention discloses a culture medium box set and method for in-vitro rapid propagation of scaevola taccada. The culture medium box set for in-vitro rapid propagation of scaevola taccada comprisesa scaevola taccada cluster bud induction culture medium, a somatic embryo and adventitious bud induction culture medium, a somatic embryo and adventitious bud proliferation and elongation culture medium, a scaevola taccada rooting induction culture medium and river sand used as a scaevola taccada tissue culture seedling transplanting medium. Moreover, an efficient scaevola taccada tissue culture seedling rapid propagation technology system utilizing the box set is also built. A basis is provided for protecting, developing and utilizing scaevola taccada resources, and a blank of scaevola taccada in the aspect of the tissue culture rapid propagation technology is filled in.

Description

Technical field [0001] The invention belongs to the technical field of plant tissue culture, and specifically relates to a culture medium kit and method for rapid propagation in vitro of P. vulgaris. Background technique [0002] Scaevola taccada (Scaevola taccada) is a semi-mangrove plant of the genus Scaevola in the Goodeniaceae family (Goodeniaceae). There are 11 genera and 400 species in the whole family. Almost all of the origins and the existing species are concentrated in Australia. Only the genus Caulis is widespread throughout the world. There are 130 species of the genus Caohaitong, and only 40 species are distributed outside Australia, most of which are scattered in tropical islands. There are two species in the southern coast of my country, namely, Scaevola hainanensis Hance and Scaevola hainanensis Hance. They are mainly distributed on open sea sands or coastal cliffs in Taiwan, Fujian, Guangdong, Guangxi and Hainan. As the dominant species in tropical and subtropi...

AI Technical Summary

Problems solved by technology

[0003] At present, the main propagation methods of Pittonia pratense are seed propagation and cutting propagation, and there is no report of realizing its propagation by means of plant tissue culture.

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